BRENDA - Enzyme Database

Urea amidolyase. I. Properties of the enzyme from Candida utilis

Roon, R.J.; Levenberg, B.; J. Biol. Chem. 247, 4107-4113 (1972)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.6
ADP
-
Cyberlindnera jadinii
6.3.4.6
Avidin
-
Saccharomyces cerevisiae
6.3.4.6
Avidin
-
Chlamydomonas reinhardtii
6.3.4.6
Avidin
-
Cyberlindnera jadinii
6.3.4.6
BaCl2
-
Cyberlindnera jadinii
6.3.4.6
CaCl2
-
Cyberlindnera jadinii
6.3.4.6
CoCl2
-
Cyberlindnera jadinii
6.3.4.6
F-
-
Cyberlindnera jadinii
6.3.4.6
HgCl2
-
Cyberlindnera jadinii
6.3.4.6
iodoacetate
-
Cyberlindnera jadinii
6.3.4.6
PCMB
-
Cyberlindnera jadinii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.6
0.1
-
Urea
-
Cyberlindnera jadinii
6.3.4.6
0.1
-
Urea
-
Saccharomyces cerevisiae
6.3.4.6
0.25
-
ATP
-
Cyberlindnera jadinii
6.3.4.6
0.5
-
HCO3-
-
Cyberlindnera jadinii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.4.6
Cs+
monovalent cation required, K+, Rb+, Cs+, or NH4+
Cyberlindnera jadinii
6.3.4.6
K+
monovalent cation required, K+, Rb+, Cs+ or NH4+
Cyberlindnera jadinii
6.3.4.6
Mg2+
divalent cation required, Mg2+ or Mn2+, maximal activity at 8 mM
Cyberlindnera jadinii
6.3.4.6
Mn2+
divalent cation required, Mg2+ or Mn2+, maximal activity at 2 mM, much less effective than Mg2+ at the optimal level
Cyberlindnera jadinii
6.3.4.6
NH4+
monovalent cation required, K+, Rb+, Cs+, or NH4+
Cyberlindnera jadinii
6.3.4.6
Rb+
monovalent cation required, K+, Rb+, Cs+, or NH4+
Cyberlindnera jadinii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.4.6
Auxenochlorella pyrenoidosa
-
grown on urea as sole nitogen source
-
6.3.4.6
Chlamydomonas reinhardtii
-
grown on urea as sole nitrogen source
-
6.3.4.6
Cyberlindnera jadinii
-
; inducible enzyme
-
6.3.4.6
Debaryomyces subglobosus
-
grown on urea as sole nitrogen source
-
6.3.4.6
Pseudochlorella pringsheimii
-
grown on urea as sole nitogen source
-
6.3.4.6
Saccharomyces cerevisiae
-
grown on urea as sole nitrogen source
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.6
ATP + acetamide + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + acetamide + HCO3-
-
1601
Saccharomyces cerevisiae
?
-
-
-
-
6.3.4.6
ATP + cyanamide + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + formamide + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + formamide + HCO3-
-
1601
Saccharomyces cerevisiae
?
-
-
-
-
6.3.4.6
ATP + hydroxyurea + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + hydroxyurea + HCO3-
-
1601
Saccharomyces cerevisiae
?
-
-
-
-
6.3.4.6
ATP + imidodicarbonic acid diamide + HCO3-
iminodicarbonic acid diamide, i.e. biuret
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + N-methylurea + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + N-methylurea + HCO3-
-
1601
Saccharomyces cerevisiae
?
-
-
-
-
6.3.4.6
ATP + propionamide + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Chlamydomonas reinhardtii
ADP + phosphate + NH3 + HCO3-
-
1601
Chlamydomonas reinhardtii
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Cyberlindnera jadinii
ADP + phosphate + NH3 + HCO3-
-
1601
Cyberlindnera jadinii
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Saccharomyces cerevisiae
ADP + phosphate + NH3 + HCO3-
-
1601
Saccharomyces cerevisiae
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Pseudochlorella pringsheimii
ADP + phosphate + NH3 + HCO3-
-
1601
Pseudochlorella pringsheimii
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Auxenochlorella pyrenoidosa
ADP + phosphate + NH3 + HCO3-
-
1601
Auxenochlorella pyrenoidosa
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Debaryomyces subglobosus
ADP + phosphate + NH3 + HCO3-
-
1601
Debaryomyces subglobosus
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.4.6
30
-
stable for short periods of time
Cyberlindnera jadinii
6.3.4.6
45
-
10 min, 50% loss of activity
Cyberlindnera jadinii
6.3.4.6
55
-
10 min, complete denaturation
Cyberlindnera jadinii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.4.6
7.8
7.9
-
Cyberlindnera jadinii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.3.4.6
7.3
8.7
about 50% of maximal activity at pH 7.3 and 8.7
Cyberlindnera jadinii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.6
ADP
-
Cyberlindnera jadinii
6.3.4.6
Avidin
-
Saccharomyces cerevisiae
6.3.4.6
Avidin
-
Chlamydomonas reinhardtii
6.3.4.6
Avidin
-
Cyberlindnera jadinii
6.3.4.6
BaCl2
-
Cyberlindnera jadinii
6.3.4.6
CaCl2
-
Cyberlindnera jadinii
6.3.4.6
CoCl2
-
Cyberlindnera jadinii
6.3.4.6
F-
-
Cyberlindnera jadinii
6.3.4.6
HgCl2
-
Cyberlindnera jadinii
6.3.4.6
iodoacetate
-
Cyberlindnera jadinii
6.3.4.6
PCMB
-
Cyberlindnera jadinii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.6
0.1
-
Urea
-
Cyberlindnera jadinii
6.3.4.6
0.1
-
Urea
-
Saccharomyces cerevisiae
6.3.4.6
0.25
-
ATP
-
Cyberlindnera jadinii
6.3.4.6
0.5
-
HCO3-
-
Cyberlindnera jadinii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.4.6
Cs+
monovalent cation required, K+, Rb+, Cs+, or NH4+
Cyberlindnera jadinii
6.3.4.6
K+
monovalent cation required, K+, Rb+, Cs+ or NH4+
Cyberlindnera jadinii
6.3.4.6
Mg2+
divalent cation required, Mg2+ or Mn2+, maximal activity at 8 mM
Cyberlindnera jadinii
6.3.4.6
Mn2+
divalent cation required, Mg2+ or Mn2+, maximal activity at 2 mM, much less effective than Mg2+ at the optimal level
Cyberlindnera jadinii
6.3.4.6
NH4+
monovalent cation required, K+, Rb+, Cs+, or NH4+
Cyberlindnera jadinii
6.3.4.6
Rb+
monovalent cation required, K+, Rb+, Cs+, or NH4+
Cyberlindnera jadinii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.6
ATP + acetamide + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + acetamide + HCO3-
-
1601
Saccharomyces cerevisiae
?
-
-
-
-
6.3.4.6
ATP + cyanamide + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + formamide + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + formamide + HCO3-
-
1601
Saccharomyces cerevisiae
?
-
-
-
-
6.3.4.6
ATP + hydroxyurea + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + hydroxyurea + HCO3-
-
1601
Saccharomyces cerevisiae
?
-
-
-
-
6.3.4.6
ATP + imidodicarbonic acid diamide + HCO3-
iminodicarbonic acid diamide, i.e. biuret
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + N-methylurea + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + N-methylurea + HCO3-
-
1601
Saccharomyces cerevisiae
?
-
-
-
-
6.3.4.6
ATP + propionamide + HCO3-
-
1601
Cyberlindnera jadinii
?
-
-
-
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Chlamydomonas reinhardtii
ADP + phosphate + NH3 + HCO3-
-
1601
Chlamydomonas reinhardtii
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Cyberlindnera jadinii
ADP + phosphate + NH3 + HCO3-
-
1601
Cyberlindnera jadinii
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Saccharomyces cerevisiae
ADP + phosphate + NH3 + HCO3-
-
1601
Saccharomyces cerevisiae
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Pseudochlorella pringsheimii
ADP + phosphate + NH3 + HCO3-
-
1601
Pseudochlorella pringsheimii
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Auxenochlorella pyrenoidosa
ADP + phosphate + NH3 + HCO3-
-
1601
Auxenochlorella pyrenoidosa
-
6.3.4.6
ATP + urea + HCO3-
-
1601
Debaryomyces subglobosus
ADP + phosphate + NH3 + HCO3-
-
1601
Debaryomyces subglobosus
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.4.6
30
-
stable for short periods of time
Cyberlindnera jadinii
6.3.4.6
45
-
10 min, 50% loss of activity
Cyberlindnera jadinii
6.3.4.6
55
-
10 min, complete denaturation
Cyberlindnera jadinii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.3.4.6
7.8
7.9
-
Cyberlindnera jadinii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.3.4.6
7.3
8.7
about 50% of maximal activity at pH 7.3 and 8.7
Cyberlindnera jadinii