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Literature summary extracted from

  • Hilger, F.; Simon, J.P.; Stalon, V.
    Yeast argininosuccinate synthetase. Purification, structural and kinetic properties (1979), Eur. J. Biochem., 94, 153-163.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.5 L-Arg non-competitive with respect to Asp and MgATP2- Saccharomyces cerevisiae
6.3.4.5 L-argininosuccinate
-
 Saccharomyces cerevisiae
6.3.4.5 phosphate competitive with respect to citrulline and Asp Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.5 49000
-
 4 * 49000, SDS-PAGE in presence of 2-mercaptoethanol Saccharomyces cerevisiae
6.3.4.5 228000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-Asp Saccharomyces cerevisiae anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source ?
-
 ?
6.3.4.5 ATP + L-citrulline + L-Asp Saccharomyces cerevisiae 1278b anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Saccharomyces cerevisiae
-
 mutant MG409
-
6.3.4.5 Saccharomyces cerevisiae 1278b
-
 mutant MG409
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.5
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.5 ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate rapid-equilibrium random mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.5 4.54
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
6.3.4.5 -20°C, stable for months Saccharomyces cerevisiae
6.3.4.5 0-4°C, without (NH4)2SO4, stable for about 2 weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-Asp r Saccharomyces cerevisiae AMP + diphosphate + L-argininosuccinate
-
 ?
6.3.4.5 ATP + L-citrulline + L-Asp r Saccharomyces cerevisiae 1278b AMP + diphosphate + L-argininosuccinate
-
 ?
6.3.4.5 ATP + L-citrulline + L-Asp anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source Saccharomyces cerevisiae ?
-
 ?
6.3.4.5 ATP + L-citrulline + L-Asp anabolic function in biosynthesis of Arg and catabolic function as the first enzyme of citrulline utilization as nitrogen source Saccharomyces cerevisiae 1278b ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.5 tetramer , 4 * 49000, SDS-PAGE in presence of 2-mercaptoethanol Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.5 6 6.5 reaction of AMP + diphosphate + L-argininosuccinate Saccharomyces cerevisiae
6.3.4.5 7.5 7.8 reaction of ATP + citrulline + L-argininosuccinate Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.4.5 5.5 7 about 60% of maximal activity at pH 5.5 and 7 Saccharomyces cerevisiae
6.3.4.5 6.5 8.5 6.5: about 55% of maximal activity, 8.5: about 70% of maximal activity, reaction of ATP + citrulline + L-argininosuccinate Saccharomyces cerevisiae