Literature summary extracted from

Liu, F.; Dong, Q.; Fromm, H.J.
Site-directed mutagenesis of the phosphate-binding consensus sequence in Escherichia coli adenylosuccinate synthetase (1992), J. Biol. Chem., 267, 2388-2392.

Protein Variants

EC Number	Protein Variants	Comment	Organism
6.3.4.4	G12V	replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme	Escherichia coli
6.3.4.4	G17V	replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme	Escherichia coli
6.3.4.4	L18R	replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.3.4.4	Escherichia coli	-	wild-type and mutant enzymes	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.3.4.4	GTP + IMP + L-Asp	-	Escherichia coli	GDP + phosphate + adenylosuccinate	-	?

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Release 2023.1 (January 2023)