EC Number | General Stability | Organism |
---|---|---|
6.3.4.3 | enzyme is unstable in phosphate buffers in the absence of high concentrations of salt | Spinacia oleracea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.3 | Ca2+ | in presence of Mg2+ | Spinacia oleracea | |
6.3.4.3 | Mn2+ | in presence of Mg2+ | Spinacia oleracea | |
6.3.4.3 | Zn2+ | in presence of Mg2+ | Spinacia oleracea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.4.3 | Ca2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Spinacia oleracea | |
6.3.4.3 | Ca2+ | Km for CaCl2: 0.21 mM | Spinacia oleracea | |
6.3.4.3 | Cs+ | activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ | Spinacia oleracea | |
6.3.4.3 | divalent metal ion | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Spinacia oleracea | |
6.3.4.3 | K+ | activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ | Spinacia oleracea | |
6.3.4.3 | Mg2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Spinacia oleracea | |
6.3.4.3 | Mg2+ | Km for MgCl2: 0.44 mM | Spinacia oleracea | |
6.3.4.3 | Mn2+ | the requirement for a divalent cation is most effectively satisfied by Mg2+. Mn2+ and Ca2+ can substitute for Mg2+ | Spinacia oleracea | |
6.3.4.3 | Mn2+ | Km for MnCl2: 0.23 mM | Spinacia oleracea | |
6.3.4.3 | monovalent cations | activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+,NH4+, Na+,Cs+ | Spinacia oleracea | |
6.3.4.3 | Na+ | activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ | Spinacia oleracea | |
6.3.4.3 | NH4+ | activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ | Spinacia oleracea | |
6.3.4.3 | Rb+ | activity is stimulated by various monovalent cations. The order of effectiveness of 10, 50 or 100 mM salts: Rb+, K+, NH4+, Na+, Cs+ | Spinacia oleracea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.4.3 | 67000 | - |
2 * 67000, SDS-PAGE | Spinacia oleracea |
6.3.4.3 | 130000 | - |
gel filtration | Spinacia oleracea |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.3 | ATP + formate + tetrahydrofolate | Spinacia oleracea | enzyme of one-carbon metabolism | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.3 | Spinacia oleracea | - |
spinach | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.3 | - |
Spinacia oleracea |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.3.4.3 | leaf | - |
Spinacia oleracea | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.4.3 | 202 | - |
- |
Spinacia oleracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.3 | ATP + formate + tetrahydrofolate | - |
Spinacia oleracea | ADP + phosphate + 10-formyltetrahydrofolate | - |
? | |
6.3.4.3 | ATP + formate + tetrahydrofolate | enzyme of one-carbon metabolism | Spinacia oleracea | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.4.3 | dimer | 2 * 67000, SDS-PAGE | Spinacia oleracea |