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Literature summary extracted from

  • Paukert, J.L.; Rabinowitz, J.C.
    Formyl-methenyl-methylenetetrahydrofolate synthetase (combined): a multifunctional protein in eukaryotic folate metabolism (1980), Methods Enzymol., 66, 616-626.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.5 Ca2+
-
 Saccharomyces cerevisiae
1.5.1.5 Mg2+
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.011
-
 NADP+
-
 Ovis aries
1.5.1.5 0.018
-
 NADP+
-
 Sus scrofa
1.5.1.5 0.022
-
 5,10-methylenetetrahydrofolate
-
 Sus scrofa
1.5.1.5 0.038
-
 5,10-methylenetetrahydrofolate
-
 Saccharomyces cerevisiae
1.5.1.5 0.039
-
 5,10-methylenetetrahydrofolate
-
 Ovis aries
1.5.1.5 0.11
-
 NADP+
-
 Saccharomyces cerevisiae
3.5.4.9 0.07
-
 10-formyltetrahydrofolate
-
 Sus scrofa
3.5.4.9 0.25
-
 5,10-methenyltetrahydrofolate
-
 Ovis aries
6.3.4.3 0.056
-
 ATP
-
 Sus scrofa
6.3.4.3 0.086
-
 ATP
-
 Ovis aries
6.3.4.3 0.12
-
 ATP
-
 Saccharomyces cerevisiae
6.3.4.3 0.23
-
 tetrahydrofolate
-
 Sus scrofa
6.3.4.3 0.46
-
 tetrahydrofolate
-
 Ovis aries
6.3.4.3 2.3
-
 tetrahydrofolate
-
 Saccharomyces cerevisiae
6.3.4.3 5.4
-
 formate
-
 Ovis aries
6.3.4.3 16
-
 formate
-
 Sus scrofa
6.3.4.3 38
-
 formate
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.5 K+ and other monovalent metal cations Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.5 100000
-
 2 * 100000, SDS-PAGE Ovis aries
1.5.1.5 150000
-
 methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, gel filtration Sus scrofa
1.5.1.5 201000
-
 methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, gel filtration Saccharomyces cerevisiae
1.5.1.5 226000
-
 methylenetetrahydrofolate dehydrogenase, formyltetrahydrofolate synthetase and methenyltetrahydrofolate cyclohydrolase combined, gel filtration Ovis aries
6.3.4.3 100000
-
 x * 100000, SDS-PAGE Sus scrofa
6.3.4.3 100000
-
 x * 100000, SDS-PAGE Saccharomyces cerevisiae
6.3.4.3 100000
-
 x * 100000, SDS-PAGE Ovis aries
6.3.4.3 150000
-
 gel filtration Sus scrofa
6.3.4.3 201000
-
 gel filtration Saccharomyces cerevisiae
6.3.4.3 226000
-
 gel filtration Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Sus scrofa
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Saccharomyces cerevisiae
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Ovis aries
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Sus scrofa
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Saccharomyces cerevisiae
-
 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O Ovis aries
-
 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Sus scrofa the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Saccharomyces cerevisiae the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate Ovis aries the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Ovis aries
-
-
-
1.5.1.5 Saccharomyces cerevisiae
-
-
-
1.5.1.5 Sus scrofa
-
-
-
3.5.4.9 Ovis aries
-
 sheep, trifunctional enzyme
-
3.5.4.9 Saccharomyces cerevisiae
-
 yeast, trifunctional enzyme
-
3.5.4.9 Sus scrofa
-
 pig, trifunctional enzyme
-
6.3.4.3 Ovis aries
-
 trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5
-
6.3.4.3 Saccharomyces cerevisiae
-
 trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5
-
6.3.4.3 Sus scrofa
-
 trifunctional enzyme with 10-formyltetrahydrofolate synthetase, EC 6.3.4.3, 5,10-methenyltetrahydrofolate cyclohydrolase, EC 3.5.4.9, and 5,10-methylenetetrahydrofolate dehydrogenase activity, EC 1.5.1.5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.5 formyltetrahydrofolate synthetase, methenyltetrahydrofolate cyclohydrolase and methylenetetrahydrofolate dehydrogenase combined Saccharomyces cerevisiae
1.5.1.5 trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase Sus scrofa
1.5.1.5 trifunctional enzyme: methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrase, formyltetrahydrofolate synthetase Ovis aries
3.5.4.9
-
 Saccharomyces cerevisiae
3.5.4.9
-
 Ovis aries
6.3.4.3
-
 Ovis aries
6.3.4.3 large-scale purification Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.5 liver
-
 Sus scrofa
-
1.5.1.5 liver
-
 Ovis aries
-
3.5.4.9 liver
-
 Sus scrofa
-
3.5.4.9 liver
-
 Ovis aries
-
6.3.4.3 liver
-
 Sus scrofa
-
6.3.4.3 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.5 additional information
-
-
 Ovis aries
3.5.4.9 0.083
-
-
 Saccharomyces cerevisiae
3.5.4.9 0.434
-
-
 Ovis aries
6.3.4.3 40
-
-
 Ovis aries
6.3.4.3 51.3
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Sus scrofa 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Saccharomyces cerevisiae 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Ovis aries 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Sus scrofa 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Saccharomyces cerevisiae 10-formyltetrahydrofolate
-
 ?
3.5.4.9 5,10-methenyltetrahydrofolate + H2O
-
 Ovis aries 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Sus scrofa ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Saccharomyces cerevisiae ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Ovis aries ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate ATP in form of MgATP2- Sus scrofa ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate ATP in form of MgATP2- Saccharomyces cerevisiae ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate ATP in form of MgATP2- Ovis aries ADP + phosphate + 10-formyltetrahydrofolate
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate Sus scrofa ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate Saccharomyces cerevisiae ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate the trifunctional enzyme is involved in the interconversion of one-carbon adducts of tetrahydrofolate Ovis aries ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.5 dimer 2 * 100000, SDS-PAGE Ovis aries
6.3.4.3 ? x * 100000, SDS-PAGE Sus scrofa
6.3.4.3 ? x * 100000, SDS-PAGE Saccharomyces cerevisiae
6.3.4.3 ? x * 100000, SDS-PAGE Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NADP+
-
 Sus scrofa
1.5.1.5 NADP+
-
 Saccharomyces cerevisiae
1.5.1.5 NADP+
-
 Ovis aries