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Literature summary extracted from

  • McDonald, T.; Breite, L.; Pangburn, K.L.W.; Hom, S.; Manser, J.; Nagel, G.M.
    Overproduction, purification, and subunit structure of Escherichia coli glycyl transfer ribonucleic acid synthetase (1980), Biochemistry, 19, 1402-1409.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.14 40000
-
 x * 40000 + x * 78000, alpha-alpha interactions contibute to the stability of the native enzyme while beta-beta interactions do not, SDS-PAGE Escherichia coli
6.1.1.14 78000
-
 x * 40000 + x * 78000, alpha-alpha interactions contibute to the stability of the native enzyme while beta-beta interactions do not, SDS-PAGE Escherichia coli
6.1.1.14 205000
-
 high speed sedimentation equilibrium measurement Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.14 Escherichia coli
-
 overproducing strain
-
6.1.1.14 Escherichia coli overproducing
-
 overproducing strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.14
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly
-
 Escherichia coli AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly
-
 Escherichia coli overproducing AMP + diphosphate + glycyl-tRNAGly
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.14 tetramer x * 40000 + x * 78000, alpha-alpha interactions contibute to the stability of the native enzyme while beta-beta interactions do not, SDS-PAGE Escherichia coli