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Literature summary extracted from

  • Lof, C.; Cohen, M.; Vermeulen, L.P.; van Roermund, C.W.T.; Wanders, R.J.A.; Meijer, A.J.
    Properties of carbamoyl-phosphate synthetase (ammonia) in rat-liver mitochondria made permeable with toluene (1983), Eur. J. Biochem., 135, 251-258.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.16 acetylglutamate activates Rattus norvegicus
6.3.4.16 acetylglutamate binding of acetylglutamate to carbamoylphosphate synthetase requires the presence of both Mg2+ and MgATP2-. The binding is inhibited by Ca2+ and N-carbamoylglutamate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.16 Ca2+ binding of acetylglutamate to carbamoylphosphate synthetase is inhibited Rattus norvegicus
6.3.4.16 Carbamoyl phosphate 50% inhibition at 13 mM Rattus norvegicus
6.3.4.16 N-carbamoylglutamate binding of acetylglutamate to carbamoylphosphate synthetase is inhibited Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.16 0.7
-
 NH3
-
 Rattus norvegicus
6.3.4.16 1.2
-
 ATP
-
 Rattus norvegicus
6.3.4.16 2
-
 NH4+
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.4.16 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.16 2 ATP + NH3 + CO2 + H2O Rattus norvegicus first enzyme involved in the biosynthesis of urea in the liver ureotelic animals ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.16 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.16 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.16 2 ATP + NH3 + CO2 + H2O first enzyme involved in the biosynthesis of urea in the liver ureotelic animals Rattus norvegicus ?
-
 ?
6.3.4.16 2 ATP + NH4+ + HCO3- ATP in form of MgATP2- Rattus norvegicus 2 ADP + phosphate + carbamoyl phosphate
-
 ?
6.3.4.16 ATP + NH4+ + CO2 + H2O
-
 Rattus norvegicus ADP + phosphate + carbamoylphosphate
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.4.16 13
-
 Carbamoyl phosphate
-
 Rattus norvegicus