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Literature summary extracted from

  • Guchhait, R.B.; Polakis, S.E.; Lane, M.D.
    Biotin carboxylase component of acetyl-CoA carboxylase from Escherichia coli (1975), Methods Enzymol., 35B, 25-31.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.14
-
 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.14 ADP
-
 Escherichia coli
6.3.4.14 GDP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.14 0.11
-
 ATP
-
 Escherichia coli
6.3.4.14 2.9
-
 HCO3-
-
 Escherichia coli
6.3.4.14 170
-
 D-(+)-biotin
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.14 Co2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli
6.3.4.14 Mg2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli
6.3.4.14 Mn2+ divalent metal ion required, Mg2+, Mn2+ or Co2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.14 95000
-
 gel filtration, analytical ultracentrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.14 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin + HCO3- ATP in form of MgATP2- Escherichia coli ADP + phosphate + carboxybiotin
-
 ?
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3-
-
 Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 ATP + D-(+)-biotin + HCO3- L-biotin is completely inactive Escherichia coli ADP + phosphate + carboxybiotin
-
 ?
6.3.4.14 GTP + biotin-carboxyl-carrier protein + HCO3-
-
 Escherichia coli GDP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 GTP + D-biotin + HCO3-
-
 Escherichia coli GDP + phosphate + carboxybiotin
-
 ?
6.3.4.14 additional information enzyme catalyzes ATP-hydrolysis in absence of biotin Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 dimer
-
 Escherichia coli
6.3.4.14 More the acetyl-CoA carboxylase system is composed of 3 components: 1. biotin carboxylase, 2. carboxyltransferase, 3. carboxylcarrier protein Escherichia coli