BRENDA - Enzyme Database show

The enzymatic synthesis of propionyl coenzyme A holocarboxylase from d-biotinyl 5'-adenylate and the apocarboxylase

Siegel, L.; Foote, J.L.; Coon, M.J.; J. Biol. Chem. 240, 1025-1031 (1965)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
6.3.4.10
loss of activity upon freezing
Oryctolagus cuniculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.10
p-chloromercuriphenylsulfonate
-
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.10
0.0000047
-
biotin
-
Oryctolagus cuniculus
6.3.4.10
0.000385
-
ATP
-
Oryctolagus cuniculus
6.3.4.10
0.0013
-
ATP
-
Oryctolagus cuniculus
6.3.4.10
0.0056
-
biotinyl-AMP
-
Oryctolagus cuniculus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.4.10
Mg2+
Mg2+ is probably required primarily for the synthesis of biotinyl-AMP from ATP and biotin, but not for the transfer of the biotin moiety from this intermediate to the apocarboxylase; required
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
Oryctolagus cuniculus
-
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.4.10
Oryctolagus cuniculus
-
-
-
6.3.4.10
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.4.10
-
Oryctolagus cuniculus
6.3.4.10
apocarboxylase
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.4.10
liver
-
Oryctolagus cuniculus
-
6.3.4.10
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.4.10
additional information
-
-
Oryctolagus cuniculus
Storage Stability
EC Number
Storage Stability
Organism
6.3.4.10
0°C, 0.5 M sucrose, stable for about 1 week
Oryctolagus cuniculus
6.3.4.10
4°C, stable for several weeks
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1394
Rattus norvegicus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1394
Rattus norvegicus
-
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
biotinyl-AMP is an intermediate
1394
Oryctolagus cuniculus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1394
Oryctolagus cuniculus
-
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1394
Oryctolagus cuniculus
?
-
-
-
-
6.3.4.10
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
19% of the activity relative to ATP
1394
Oryctolagus cuniculus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
6.3.4.10
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
11% of the activity relative to ATP
1394
Oryctolagus cuniculus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
6.3.4.10
UTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
24% of the activity relative to ATP
1394
Oryctolagus cuniculus
UMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.4.10
53
-
10-19% loss of activity after 1 min, 73-76% loss of activity after 5 min, 91-93% loss of activity after 15 min; 10% loss of activity after 1 min, 63% loss of activity after 5 min, 91% loss of activity after 15 min
Oryctolagus cuniculus
General Stability (protein specific)
EC Number
General Stability
Organism
6.3.4.10
loss of activity upon freezing
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.3.4.10
p-chloromercuriphenylsulfonate
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.4.10
0.0000047
-
biotin
-
Oryctolagus cuniculus
6.3.4.10
0.000385
-
ATP
-
Oryctolagus cuniculus
6.3.4.10
0.0013
-
ATP
-
Oryctolagus cuniculus
6.3.4.10
0.0056
-
biotinyl-AMP
-
Oryctolagus cuniculus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.3.4.10
Mg2+
Mg2+ is probably required primarily for the synthesis of biotinyl-AMP from ATP and biotin, but not for the transfer of the biotin moiety from this intermediate to the apocarboxylase; required
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
Oryctolagus cuniculus
-
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.4.10
-
Oryctolagus cuniculus
6.3.4.10
apocarboxylase
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.3.4.10
liver
-
Oryctolagus cuniculus
-
6.3.4.10
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.3.4.10
additional information
-
-
Oryctolagus cuniculus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
6.3.4.10
0°C, 0.5 M sucrose, stable for about 1 week
Oryctolagus cuniculus
6.3.4.10
4°C, stable for several weeks
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1394
Rattus norvegicus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1394
Rattus norvegicus
-
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
biotinyl-AMP is an intermediate
1394
Oryctolagus cuniculus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1394
Oryctolagus cuniculus
-
6.3.4.10
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1394
Oryctolagus cuniculus
?
-
-
-
-
6.3.4.10
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
19% of the activity relative to ATP
1394
Oryctolagus cuniculus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
6.3.4.10
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
11% of the activity relative to ATP
1394
Oryctolagus cuniculus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
6.3.4.10
UTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
24% of the activity relative to ATP
1394
Oryctolagus cuniculus
UMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
6.3.4.10
53
-
10-19% loss of activity after 1 min, 73-76% loss of activity after 5 min, 91-93% loss of activity after 15 min; 10% loss of activity after 1 min, 63% loss of activity after 5 min, 91% loss of activity after 15 min
Oryctolagus cuniculus