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Literature summary extracted from
- Thermopsin (1995), Methods Enzymol., 248, 156-168.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.42 | expression in Escherichia coli | Sulfolobus acidocaldarius |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.42 | 1,2-epoxy-3-(p-nitrophenoxy)propane | - |
Sulfolobus acidocaldarius |  |
| 3.4.23.42 | diazoacetyl-DL-norleucine | - |
Sulfolobus acidocaldarius | |
| 3.4.23.42 | Hg(CH3COO)2 | - |
Sulfolobus acidocaldarius | |
| 3.4.23.42 | pepstatin | - |
Sulfolobus acidocaldarius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.23.42 | 0.053 | - |
Lys-Pro-Ala-Glu-Phe-(4-nitro)Phe-Ala-Leu | - |
Sulfolobus acidocaldarius | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.23.42 | cell wall | attached to | Sulfolobus acidocaldarius | 5618 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.42 | Protein + H2O | Sulfolobus acidocaldarius | in growth medium | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.42 | Sulfolobus acidocaldarius | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.42 | - |
Sulfolobus acidocaldarius |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.42 | additional information | - |
- |
Sulfolobus acidocaldarius |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.23.42 | 4°C, several months | Sulfolobus acidocaldarius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.42 | Hemoglobin + H2O | - |
Sulfolobus acidocaldarius | ? | - |
? | |
| 3.4.23.42 | Lys-Pro-Ala-Glu-Phe-(4-nitro)Phe-Ala-Leu + H2O | - |
Sulfolobus acidocaldarius | Lys-Pro-Ala-Glu-Phe + (4-nitro)Phe-Ala-Leu | - |
? | |
| 3.4.23.42 | oxidized insulin B + H2O | following bonds are hydrolyzed: Leu-Val, Leu-Tyr, Phe-Phe, Phe-Tyr, Tyr-Thr | Sulfolobus acidocaldarius | ? | - |
? | |
| 3.4.23.42 | Protein + H2O | in growth medium | Sulfolobus acidocaldarius | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.42 | 90 | - |
- |
Sulfolobus acidocaldarius |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.42 | 2 | - |
- |
Sulfolobus acidocaldarius |
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