EC Number | General Stability | Organism |
---|---|---|
3.13.2.1 | freezing and thawing in absence of glycerol causes formation of multimers, low thiol concentrations and exposure to atmospheric O2 fortifies aggregate formation | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.13.2.1 | 8-(3-aminopropylamino)adenosine | - |
Rattus norvegicus | |
3.13.2.1 | adenosine | irreversible | Rattus norvegicus | |
3.13.2.1 | S-adenosyl-L-homocysteine | - |
Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.13.2.1 | 0.0006 | - |
adenosine | - |
Rattus norvegicus | |
3.13.2.1 | 0.0009 | - |
S-adenosyl-L-homocysteine | - |
Rattus norvegicus | |
3.13.2.1 | 0.06 | - |
L-homocysteine | - |
Rattus norvegicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 47000 | - |
x * 47000, SDS-PAGE | Rattus norvegicus |
3.13.2.1 | 210000 | - |
gel filtration | Rattus norvegicus |
3.13.2.1 | 220000 | 229000 | gel electrophoresis | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.2.1 | S-adenosyl-L-homocysteine + H2O | Rattus norvegicus | - |
adenosine + L-homocysteine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.13.2.1 | Rattus norvegicus | - |
Wistar rats | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
3.13.2.1 | 2-mercaptoethanol prevents aggregation of enzyme | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.13.2.1 | 135fold to homogeneity | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.13.2.1 | S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine | reaction equilibrium is in vitro strongly in direction of synthesis of S-adenosyl-L-homocysteine | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.13.2.1 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.13.2.1 | 5.95 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
3.13.2.1 | -80°C, with 20% glycerol, stable for 8 months | Rattus norvegicus |
3.13.2.1 | 0°C, stable in 100 mM potassium phosphate containing 5 mM dithiothreitol, with 1 mM dithiothreitol the enzyme is stable for 1 month but losing activity thereafter rapidly | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.13.2.1 | S-adenosyl-L-homocysteine + H2O | - |
Rattus norvegicus | adenosine + L-homocysteine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.13.2.1 | ? | x * 47000, SDS-PAGE | Rattus norvegicus |