BRENDA - Enzyme Database show

Purification and properties of nucleoside hydrolase from Pseudomonas fluorescens

Terada, M.; Tatibana, M.; Hayaishi, O.; J. Biol. Chem. 242, 5578-5585 (1967)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.8
adenosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
3.2.2.8
AgCl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
CaCl2
at 1 mM, inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
CdCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
CoCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
CuCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
cytidine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
3.2.2.8
EDTA
at 1 mM: inhibits uridine hydrolysis by 50%
Pseudomonas fluorescens
3.2.2.8
FeCl3
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
FeSO4
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
HgCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
Inosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
3.2.2.8
MgCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
MnSO4
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
NH4Cl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
ZnCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.8
0.2
-
Inosine
pH 8.5
Pseudomonas fluorescens
3.2.2.8
0.5
-
adenosine
pH 8.5
Pseudomonas fluorescens
3.2.2.8
0.5
-
Inosine
-
Pseudomonas fluorescens
3.2.2.8
0.6
-
guanosine
-
Pseudomonas fluorescens
3.2.2.8
0.8
-
uridine
-
Pseudomonas fluorescens
3.2.2.8
1
-
cytidine
pH 8.5
Pseudomonas fluorescens
3.2.2.8
2.5
-
5-Bromouridine
-
Pseudomonas fluorescens
3.2.2.8
6.5
-
Xanthosine
-
Pseudomonas fluorescens
3.2.2.8
7.5
-
1-beta-D-ribofuranosylthymine
-
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.8
N-D-ribosylpyrimidine
Pseudomonas fluorescens
-
pyrimidine + D-ribose
-
Pseudomonas fluorescens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.8
Pseudomonas fluorescens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.2.8
partial purification, 300fold
Pseudomonas fluorescens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.8
272
-
at 37°C
Pseudomonas fluorescens
Storage Stability
EC Number
Storage Stability
Organism
3.2.2.8
-15°C, 1-3 mg of protein per ml, stable for 6 months
Pseudomonas fluorescens
3.2.2.8
4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month
Pseudomonas fluorescens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.8
1-beta-D-ribofuranosylnicotinamide + H2O
-
137117
Pseudomonas fluorescens
nicotinamide + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
1-beta-D-ribofuranosylthymine + H2O
-
137117
Pseudomonas fluorescens
D-ribose + thymine
-
137117
Pseudomonas fluorescens
?
3.2.2.8
4-amino-5-imidazolecarboxamide ribonucleotide + H2O
-
137117
Pseudomonas fluorescens
4-amino-5-imidazolecarboxamide + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
5-bromouridine + H2O
-
137117
Pseudomonas fluorescens
5-bromouracil + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
6-azauridine + H2O
poor substrate
137117
Pseudomonas fluorescens
6-azauracil + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
adenosine + H2O
-
137117
Pseudomonas fluorescens
adenine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
cytidine + H2O
-
137117
Pseudomonas fluorescens
cytosine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
guanosine + H2O
-
137117
Pseudomonas fluorescens
guanine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
imidazoleacetic acid ribonucleotide + H2O
-
137117
Pseudomonas fluorescens
imidazoleacetic acid + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
inosine + H2O
-
137117
Pseudomonas fluorescens
hypoxanthine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
N-D-ribosylpyrimidine
-
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
N-D-ribosylpyrimidine + H2O
-
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
purine D-ribonucleosides + H2O
prefers pyrimidines
137117
Pseudomonas fluorescens
purine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
tubercidin + H2O
poor substrate
137117
Pseudomonas fluorescens
1-deazaadenine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
uridine + H2O
-
137117
Pseudomonas fluorescens
uracil + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
xanthosine + H2O
-
137117
Pseudomonas fluorescens
xanthine + D-ribose
-
137117
Pseudomonas fluorescens
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.8
45
-
for 10 min at pH 5.0, 50% of activity remains; for 10 min at pH 7.0-9.2, more than 90% of activity remains
Pseudomonas fluorescens
3.2.2.8
60
-
for 15 min, 60% denaturation
Pseudomonas fluorescens
3.2.2.8
70
-
for 5 min at pH 8.0, complete inactivation
Pseudomonas fluorescens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.8
6
-
substrate: inosine
Pseudomonas fluorescens
3.2.2.8
6.5
-
substrate: guanosine
Pseudomonas fluorescens
3.2.2.8
7
-
substrate: xanthosine
Pseudomonas fluorescens
3.2.2.8
8
-
substrate: 1-beta-D-ribofuranosylthymine
Pseudomonas fluorescens
3.2.2.8
8.5
-
substrate: cytidine, uridine, 5-bromouridine and adenosine
Pseudomonas fluorescens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.8
adenosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
3.2.2.8
AgCl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
CaCl2
at 1 mM, inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
CdCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
CoCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
CuCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
cytidine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
3.2.2.8
EDTA
at 1 mM: inhibits uridine hydrolysis by 50%
Pseudomonas fluorescens
3.2.2.8
FeCl3
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
FeSO4
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
HgCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
Inosine
at 6.25 mM, competitive inhibitor for uridine hydrolysis
Pseudomonas fluorescens
3.2.2.8
MgCl2
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
MnSO4
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
NH4Cl
at 1 mM: inhibition less than 10%
Pseudomonas fluorescens
3.2.2.8
ZnCl2
at 1 mM: inhibition ranging from 40-95%, at 0.01 mM: inhibition less than 10%
Pseudomonas fluorescens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.8
0.2
-
Inosine
pH 8.5
Pseudomonas fluorescens
3.2.2.8
0.5
-
adenosine
pH 8.5
Pseudomonas fluorescens
3.2.2.8
0.5
-
Inosine
-
Pseudomonas fluorescens
3.2.2.8
0.6
-
guanosine
-
Pseudomonas fluorescens
3.2.2.8
0.8
-
uridine
-
Pseudomonas fluorescens
3.2.2.8
1
-
cytidine
pH 8.5
Pseudomonas fluorescens
3.2.2.8
2.5
-
5-Bromouridine
-
Pseudomonas fluorescens
3.2.2.8
6.5
-
Xanthosine
-
Pseudomonas fluorescens
3.2.2.8
7.5
-
1-beta-D-ribofuranosylthymine
-
Pseudomonas fluorescens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.8
N-D-ribosylpyrimidine
Pseudomonas fluorescens
-
pyrimidine + D-ribose
-
Pseudomonas fluorescens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.8
partial purification, 300fold
Pseudomonas fluorescens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.2.8
272
-
at 37°C
Pseudomonas fluorescens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.2.8
-15°C, 1-3 mg of protein per ml, stable for 6 months
Pseudomonas fluorescens
3.2.2.8
4°C, 0.032 mg protein per ml, over 70% of activity remained after 1 month
Pseudomonas fluorescens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.8
1-beta-D-ribofuranosylnicotinamide + H2O
-
137117
Pseudomonas fluorescens
nicotinamide + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
1-beta-D-ribofuranosylthymine + H2O
-
137117
Pseudomonas fluorescens
D-ribose + thymine
-
137117
Pseudomonas fluorescens
?
3.2.2.8
4-amino-5-imidazolecarboxamide ribonucleotide + H2O
-
137117
Pseudomonas fluorescens
4-amino-5-imidazolecarboxamide + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
5-bromouridine + H2O
-
137117
Pseudomonas fluorescens
5-bromouracil + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
6-azauridine + H2O
poor substrate
137117
Pseudomonas fluorescens
6-azauracil + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
adenosine + H2O
-
137117
Pseudomonas fluorescens
adenine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
cytidine + H2O
-
137117
Pseudomonas fluorescens
cytosine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
guanosine + H2O
-
137117
Pseudomonas fluorescens
guanine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
imidazoleacetic acid ribonucleotide + H2O
-
137117
Pseudomonas fluorescens
imidazoleacetic acid + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
inosine + H2O
-
137117
Pseudomonas fluorescens
hypoxanthine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
N-D-ribosylpyrimidine
-
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
N-D-ribosylpyrimidine + H2O
-
137117
Pseudomonas fluorescens
pyrimidine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
purine D-ribonucleosides + H2O
prefers pyrimidines
137117
Pseudomonas fluorescens
purine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
tubercidin + H2O
poor substrate
137117
Pseudomonas fluorescens
1-deazaadenine + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
uridine + H2O
-
137117
Pseudomonas fluorescens
uracil + D-ribose
-
137117
Pseudomonas fluorescens
?
3.2.2.8
xanthosine + H2O
-
137117
Pseudomonas fluorescens
xanthine + D-ribose
-
137117
Pseudomonas fluorescens
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.8
45
-
for 10 min at pH 5.0, 50% of activity remains; for 10 min at pH 7.0-9.2, more than 90% of activity remains
Pseudomonas fluorescens
3.2.2.8
60
-
for 15 min, 60% denaturation
Pseudomonas fluorescens
3.2.2.8
70
-
for 5 min at pH 8.0, complete inactivation
Pseudomonas fluorescens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.8
6
-
substrate: inosine
Pseudomonas fluorescens
3.2.2.8
6.5
-
substrate: guanosine
Pseudomonas fluorescens
3.2.2.8
7
-
substrate: xanthosine
Pseudomonas fluorescens
3.2.2.8
8
-
substrate: 1-beta-D-ribofuranosylthymine
Pseudomonas fluorescens
3.2.2.8
8.5
-
substrate: cytidine, uridine, 5-bromouridine and adenosine
Pseudomonas fluorescens