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Literature summary extracted from

  • Negishi, O.; Ozawa, T.; Imagawa, H.
    N-methyl nucleosidase from tea leaves (1988), Agric. Biol. Chem., 52, 169-175.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.25 7-Methylhypoxanthine 50% inhibition at 5 mM, no inhibition at 1 mM Camellia sinensis
3.2.2.25 7-methylxanthine 40% inhibition at 5 mM, no inhibition at 1 mM Camellia sinensis
3.2.2.25 Ca2+ slight inhibition Camellia sinensis
3.2.2.25 Cu2+ slight inhibition Camellia sinensis
3.2.2.25 EDTA 90 inhibition at 1 mM Camellia sinensis
3.2.2.25 additional information no inhibitin by Mg2+, Fe2+, Co2+, NaF, and iodoacetate Camellia sinensis
3.2.2.25 PCMB 80% inhibition at 0.5 mM Camellia sinensis
3.2.2.25 Zn2+ slight inhibition Camellia sinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.25 55000
-
 gel filtration Camellia sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.7 adenosine + H2O Camellia sinensis component of the purine salvage pathway 6-aminopurine + D-ribofuranose
-
 ?
3.2.2.7 adenosine + H2O Camellia sinensis 6-aminopurine-9-beta-D-ribofuranoside 6-aminopurine + D-ribofuranose
-
 ?
3.2.2.25 7-methylxanthosine + H2O Camellia sinensis second step in caffeine biosynthesis, pathway overview 7-methylxanthine + D-ribose
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.7 Camellia sinensis
-
 tea
-
3.2.2.25 Camellia sinensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.7 partial Camellia sinensis
3.2.2.25 native enzyme from leaves, separated from adenosine nucleosidase, EC 3.2.2.7, by anion exchange chromatography, followed by hydroxylapatite chromatography and gel filtration, to homogeneity Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.7 leaf
-
 Camellia sinensis
-
3.2.2.25 leaf
-
 Camellia sinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.7 4
-
-
 Camellia sinensis
3.2.2.25 0.00656
-
 partially purified enzyme, after anion exchange chromatography Camellia sinensis
3.2.2.25 0.0294
-
 purified enzyme Camellia sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.7 7-methyl-6-aminopurine-9-beta-D-ribofuranoside + H2O 5fold faster hydrolysis compared to adenosine Camellia sinensis 7-methyl-6-aminopurine + D-ribofuranose
-
 ?
3.2.2.7 adenosine + H2O component of the purine salvage pathway Camellia sinensis 6-aminopurine + D-ribofuranose
-
 ?
3.2.2.7 adenosine + H2O 6-aminopurine-9-beta-D-ribofuranoside Camellia sinensis 6-aminopurine + D-ribofuranose
-
 ?
3.2.2.25 1-methyladenosine + H2O 22% of the activity with 7-methylxanthosine, substrate as 7-methyladenosine perchlorate Camellia sinensis 1-methyladenine + D-ribose
-
 ir
3.2.2.25 1-methylinosine + H2O 9% of the activity with 7-methylxanthosine Camellia sinensis 1-methylhypoxanthine + D-ribose
-
 ir
3.2.2.25 1-methylxanthosine + H2O 12% of the activity with 7-methylxanthosine Camellia sinensis 1-methylxanthine + D-ribose
-
 ir
3.2.2.25 3-methyladenosine + H2O 178% of the activity with 7-methylxanthosine, substrate as 3-methyladenosine p-toluenesulfonate Camellia sinensis 3-methyladenine + D-ribose
-
 ir
3.2.2.25 3-methylguanosine + H2O 38% of the activity with 7-methylxanthosine Camellia sinensis 3-methylguanine + D-ribose
-
 ir
3.2.2.25 3-methylinosine + H2O 128% of the activity with 7-methylxanthosine Camellia sinensis 3-methylhypoxanthine + D-ribose
-
 ir
3.2.2.25 3-methylxanthosine + H2O 198% of the activity with 7-methylxanthosine Camellia sinensis 3-methylxanthine + D-ribose
-
 ir
3.2.2.25 7-methyladenosine + H2O 13% of the activity with 7-methylxanthosine Camellia sinensis 7-methyladenine + D-ribose
-
 ir
3.2.2.25 7-methylguanosine + H2O 189% of the activity with 7-methylxanthosine Camellia sinensis 7-methylguanine + D-ribose
-
 ir
3.2.2.25 7-methylinosine + H2O 121% of the activity with 7-methylxanthosine Camellia sinensis 7-methylhypoxanthine + D-ribose
-
 ir
3.2.2.25 7-methylxanthosine + H2O second step in caffeine biosynthesis, pathway overview Camellia sinensis 7-methylxanthine + D-ribose
-
 ir
3.2.2.25 7-methylxanthosine + H2O the reverse reaction is catalyzed by a nucleoside phosphorylase using ribose-1-phosphate, overview Camellia sinensis 7-methylxanthine + D-ribose
-
 ir
3.2.2.25 additional information substrate specificity, overview, no activity with 1-methylguanosine and guanosine, poor activity with inosine and adenosine Camellia sinensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.25 N-MeNase
-
 Camellia sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.25 37
-
 assay at Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.25 8 8.5
-
 Camellia sinensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.2.25 additional information
-
 the purified enzyme is unstable in acidic medium Camellia sinensis