BRENDA - Enzyme Database show

Purification and characterization of NAD glycohydrolase from rabbit erythrocytes

Kim, U.H.; Kim, M.K.; Kim, J.S.; Han, M.K.; Park, B.H.; Kim, H.R.; Arch. Biochem. Biophys. 305, 147-152 (1993)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
ADPribose
-
Oryctolagus cuniculus
3.2.2.5
isoniazid
-
Oryctolagus cuniculus
3.2.2.5
nicotinamide
-
Oryctolagus cuniculus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.5
0.043
-
NAD+
-
Oryctolagus cuniculus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.5
63000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
NAD+ + H2O
Oryctolagus cuniculus
-
ADPribose + nicotinamide
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.2.5
Oryctolagus cuniculus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.2.5
glycoprotein
-
Oryctolagus cuniculus
Purification (Commentary)
EC Number
Commentary
Organism
3.2.2.5
-
Oryctolagus cuniculus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
erythrocyte
-
Oryctolagus cuniculus
-
Storage Stability
EC Number
Storage Stability
Organism
3.2.2.5
-20°C, 6 months, phosphate buffer, 30%, w/v glycerol
Oryctolagus cuniculus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
3-acetylpyridine hypoxanthine dinucleotide + H2O
-
137094
Oryctolagus cuniculus
?
-
-
-
?
3.2.2.5
NAD+ + H2O
-
137094
Oryctolagus cuniculus
ADPribose + nicotinamide
-
-
-
-
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.5
50
-
10 min, loss of 50% of activity
Oryctolagus cuniculus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
7
-
broad pH-optimum
Oryctolagus cuniculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.2.5
ADPribose
-
Oryctolagus cuniculus
3.2.2.5
isoniazid
-
Oryctolagus cuniculus
3.2.2.5
nicotinamide
-
Oryctolagus cuniculus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.2.5
0.043
-
NAD+
-
Oryctolagus cuniculus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.2.5
63000
-
gel filtration
Oryctolagus cuniculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.2.5
NAD+ + H2O
Oryctolagus cuniculus
-
ADPribose + nicotinamide
-
-
-
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.2.5
glycoprotein
-
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.2.5
-
Oryctolagus cuniculus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.2.5
erythrocyte
-
Oryctolagus cuniculus
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.2.5
-20°C, 6 months, phosphate buffer, 30%, w/v glycerol
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.2.5
3-acetylpyridine hypoxanthine dinucleotide + H2O
-
137094
Oryctolagus cuniculus
?
-
-
-
?
3.2.2.5
NAD+ + H2O
-
137094
Oryctolagus cuniculus
ADPribose + nicotinamide
-
-
-
-
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.2.5
50
-
10 min, loss of 50% of activity
Oryctolagus cuniculus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.2.5
7
-
broad pH-optimum
Oryctolagus cuniculus