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Literature summary extracted from
- Bacillus subtilis NADase and its specific protein inhibitor (1986), Methods Enzymol., 66, 137-144.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.5 | 3-acetylpyridine | - |
Bacillus subtilis |  |
| 3.2.2.5 | additional information | nicotinylhydroxamic acid derivates of NAD+ | Bacillus subtilis | |
| 3.2.2.5 | thioNAD+ | - |
Bacillus subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.2.5 | 0.55 | - |
NAD+ | - |
Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.5 | 26200 | - |
ultracentrifugation | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.5 | NAD+ + H2O | Bacillus subtilis | - |
ADPribose + nicotinamide | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.5 | Bacillus subtilis | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.2.5 | glycoprotein | 52.8% carbohydrate | Bacillus subtilis |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.5 | - |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.5 | 850 | - |
- |
Bacillus subtilis |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.2.5 | -20°C, over 1 year | Bacillus subtilis |
| 3.2.2.5 | 4°C, several months | Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.5 | NAD+ + H2O | - |
Bacillus subtilis | ADPribose + nicotinamide | - |
ir | |
| 3.2.2.5 | NADP+ + H2O | - |
Bacillus subtilis | phospho-ADPribose + nicotinamide | - |
ir | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.5 | additional information | - |
stable to heat, activation of enzyme through boiling | Bacillus subtilis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.2.5 | 0.358 | - |
NAD+ | - |
Bacillus subtilis | |
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