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Literature summary extracted from

  • Yoshino, M.; Tsukada, T.; Tsushima, K.
    Inosine nucleosidase from Azotobacter vinelandii. Purification and properties (1978), Arch. Microbiol., 119, 59-64.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.2 adenine partial noncompetitive Azotobacter vinelandii
3.2.2.2 hypoxanthine partial noncompetitive Azotobacter vinelandii
3.2.2.2 nucleoside diphosphates less effective inhibitor compared to nucleoside triphosphates, non competitive inhibition Azotobacter vinelandii
3.2.2.2 nucleoside monophosphates slight inhibition or no effect Azotobacter vinelandii
3.2.2.2 nucleoside triphosphates most potent inhibitor, non competitive inhibition Azotobacter vinelandii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.2 0.65
-
 Inosine pH 7.1 Azotobacter vinelandii
3.2.2.2 0.85
-
 Inosine pH 9.0 Azotobacter vinelandii
3.2.2.2 1.2
-
 Xanthosine pH 7.1 Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.2 70000
-
 gel filtration Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.2 inosine + H2O Azotobacter vinelandii
-
 hypoxanthine + D-ribose
-
 ?
3.2.2.2 inosine + H2O Azotobacter vinelandii 0
-
 hypoxanthine + D-ribose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.2 Azotobacter vinelandii
-
-
-
3.2.2.2 Azotobacter vinelandii 0
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.2 highly purified, 233fold Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.2 2.33
-
-
 Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.2 adenosine + H2O
-
 Azotobacter vinelandii adenine + D-ribose
-
 ?
3.2.2.2 adenosine + H2O
-
 Azotobacter vinelandii 0 adenine + D-ribose
-
 ?
3.2.2.2 guanosine + H2O
-
 Azotobacter vinelandii guanine + D-ribose
-
 ?
3.2.2.2 guanosine + H2O
-
 Azotobacter vinelandii 0 guanine + D-ribose
-
 ?
3.2.2.2 inosine + H2O
-
 Azotobacter vinelandii hypoxanthine + D-ribose
-
 ?
3.2.2.2 inosine + H2O
-
 Azotobacter vinelandii 0 hypoxanthine + D-ribose
-
 ?
3.2.2.2 xanthosine + H2O
-
 Azotobacter vinelandii xanthine + D-ribose
-
 ?
3.2.2.2 xanthosine + H2O
-
 Azotobacter vinelandii 0 xanthine + D-ribose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.2 7
-
 substrate: xanthosine Azotobacter vinelandii
3.2.2.2 9
-
 substrate: inosine Azotobacter vinelandii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.2 7 9.5
-
 Azotobacter vinelandii
3.2.2.2 9
-
 no xanthosine hydrolysis above this temperature Azotobacter vinelandii