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Literature summary extracted from

  • Thompson, J.; Ruvinov, S.B.; Freedberg, D.I.; Hall, B.G.
    Cellobiose-6-phosphate hydrolase (CelF) of Escherichia coli: Characterization and Assignment to the unsual family 4 of glycosylhydrolases (1999), J. Bacteriol., 181, 7339-7345.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.86 0.3
-
 4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside
-
 Escherichia coli
3.2.1.86 0.35
-
 arbutin 6-phosphate
-
 Escherichia coli
3.2.1.86 0.44
-
 Salicin 6-phosphate
-
 Escherichia coli
3.2.1.86 0.44
-
 4-nitrophenyl beta-D-glucopyranoside 6-phosphate
-
 Escherichia coli
3.2.1.86 1.3
-
 cellobiose 6-phosphate
-
 Escherichia coli
3.2.1.86 2.22
-
 methyl beta-D-glucoside 6-phosphate
-
 Escherichia coli
3.2.1.86 12.5
-
 6-O-(6-O-phosphono-beta-D-glucopyranosyl)-beta-D-glucopyranose
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.86 21000
-
 gel filtration Escherichia coli
3.2.1.86 50000
-
 SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.86 Escherichia coli P17411
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.86 4-methyl-2-oxo-2H-chromen-7-yl 6-O-phosphono-beta-D-glucopyranoside + H2O
-
 Escherichia coli ?
-
 ?
3.2.1.86 4-nitrophenyl beta-D-glucopyranoside 6-phosphate + H2O
-
 Escherichia coli 4-nitrophenol + beta-D-glucopyranose 6-phosphate
-
 ?
3.2.1.86 arbutin 6-phosphate + H2O
-
 Escherichia coli hydroquinone + D-glucose 6-phosphate
-
 ?
3.2.1.86 cellobiose 6-phosphate + H2O
-
 Escherichia coli D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.86 gentiobiose 6-phosphate + H2O
-
 Escherichia coli D-glucose 6-phosphate + D-glucose
-
 ?
3.2.1.86 methyl beta-glucoside 6-phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
 Escherichia coli p-nitrophenol + D-glucopyranose 6-phosphate
-
 ?
3.2.1.86 salicin 6-phosphate + H2O
-
 Escherichia coli 2-hydroxymethylphenol + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.86 tetramer 4 * 50000 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.86 34 38
-
 Escherichia coli