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Literature summary extracted from

  • Wilson, G.; Fox, C.F.
    The beta-glucoside system of Escherichia coli. IV. Purification and properties of phospho-beta-glucosidases A and B (1974), J. Biol. Chem., 249, 5586-5598.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.86 2'-AMP
-
 Escherichia coli
3.2.1.86 3',5'-AMP
-
 Escherichia coli
3.2.1.86 3'-AMP
-
 Escherichia coli
3.2.1.86 AMP
-
 Escherichia coli
3.2.1.86 ATP
-
 Escherichia coli
3.2.1.86 CMP
-
 Escherichia coli
3.2.1.86 DL-alpha-glycerol-phosphate
-
 Escherichia coli
3.2.1.86 fructose-1,6-diphosphate
-
 Escherichia coli
3.2.1.86 NADH
-
 Escherichia coli
3.2.1.86 phosphoenolpyruvate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.86 0.11
-
 4-nitrothiophenyl beta-D-glucoside 6-phosphate isoenzyme B Escherichia coli
3.2.1.86 0.18
-
 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate isoenzyme B Escherichia coli
3.2.1.86 0.19
-
 methyl beta-D-glucoside 6-phosphate isoenzyme B Escherichia coli
3.2.1.86 0.25
-
 2-nitrophenyl beta-D-galactoside 6-phosphate isoenzyme B Escherichia coli
3.2.1.86 0.28
-
 methyl beta-D-glucoside 6-phosphate isoenzyme A Escherichia coli
3.2.1.86 0.33
-
 p-nitrophenyl-D-beta-D-glucopyranoside 6-phosphate isoenzyme A Escherichia coli
3.2.1.86 0.46
-
 phenyl beta-D-glucoside 6-phosphate isoenzyme B Escherichia coli
3.2.1.86 0.51
-
 Salicin 6-phosphate isoenzyme B Escherichia coli
3.2.1.86 0.6
-
 phenyl beta-D-glucoside 6-phosphate isoenzyme A Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.86 65000
-
 isoenzyme B, SDS-PAGE Escherichia coli
3.2.1.86 68000
-
 isoenzyme A, SDS-PAGE Escherichia coli
3.2.1.86 132000
-
 isoenzyme B, molecular sieve chromatography together with sedimentation velocity measurement Escherichia coli
3.2.1.86 142000
-
 isoenzyme A, molecular sieve chromatography together with sedimentation velocity measurement Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.86 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.86 63 fold Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.86 85
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
3.2.1.86 isoenzyme A, 4°C, 1 year, sterile starting buffer, 0% loss in activity Escherichia coli
3.2.1.86 isoenzyme B, -20°C, 2 months, sterile starting buffer, 50% loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.86 2-nitrophenyl beta-D-galactoside 6-phosphate + H2O
-
 Escherichia coli 2-nitrophenol + beta-D-galactose 6-phosphate
-
 ?
3.2.1.86 4-nitrothiophenyl beta-D-glucoside 6-phosphate + H2O
-
 Escherichia coli 4-nitrothiophenol + beta-D-glucose 6-phosphate
-
 ?
3.2.1.86 methyl beta-glucoside 6-phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.2.1.86 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Escherichia coli o-nitrophenol + D-galactose 6-phosphate
-
 ?
3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O isoenzyme B Escherichia coli p-nitrophenol + D-glucopyranose 6-phosphate
-
 ?
3.2.1.86 p-nitrothiophenyl beta-glucoside 6-phosphate + H2O
-
 Escherichia coli ?
-
 ?
3.2.1.86 phenyl beta-D-glucoside 6-phosphate + H2O
-
 Escherichia coli phenol + glucose 6-phosphate
-
 ?
3.2.1.86 salicin 6-phosphate + H2O isoenzyme B Escherichia coli 2-hydroxymethylphenol + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.86 dimer homodimer, 2 * 68000 isoenzyme A, 2 * 65000 isoenzyme B, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.86 10 28 isoenzyme B Escherichia coli
3.2.1.86 25 45 isoenzyme A Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.86 6.3
-
 isoenzyme A, p-nitrophenyl beta-D-glucospyranoside Escherichia coli
3.2.1.86 7 9 isoenzyme B, p-nitrophenyl beta-D-glucospyranoside Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.86 4 10
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.86 4 10 for more than 1 h Escherichia coli