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Literature summary extracted from
- Isolation of a homogeneous glucosidase II from pig kidney microsomes (1984), Eur. J. Biochem., 141, 149-156.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.207 | isomaltose | lowering Km, 142% activation at 50 mM | Sus scrofa |  |
| 3.2.1.207 | mannose | increasing Vmax, 172% activation at 10 mM | Sus scrofa | |
| 3.2.1.207 | starch | increasing Vmax, 160% activation at 7 mg/ml | Sus scrofa | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.207 | glycerol, stabilization | Sus scrofa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.207 | maltose | - |
Sus scrofa | |
| 3.2.1.207 | Tris | - |
Sus scrofa | |
| 3.2.1.207 | turanose | - |
Sus scrofa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.207 | 0.019 | - |
4-methylumbelliferyl alpha-D-glucopyranoside | - |
Sus scrofa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.207 | endoplasmic reticulum | lumen | Sus scrofa | 5783 | - |
| 3.2.1.207 | microsome | - |
Sus scrofa | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 100000 | - |
4 * 100000, SDS-PAGE | Sus scrofa |
| 3.2.1.207 | 400000 | - |
non denaturing PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.207 | Glc2Man9GlcNAc2 + H2O | Sus scrofa | - |
Man9GlcNAc2 + alpha-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.207 | Sus scrofa | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.207 | glycoprotein | - |
Sus scrofa |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.207 | - |
Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.207 | kidney | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 0.151 | - |
4-methylumbelliferyl alpha-D-glucopyranoside as substrate | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.207 | 4-methylumbelliferyl-alpha-D-glucopyranoside + H2O | - |
Sus scrofa | 4-methylumbelliferone + alpha-D-glucose | - |
? | |
| 3.2.1.207 | Glc2Man9GlcNAc2 + 2 H2O | - |
Sus scrofa | Man9GlcNAc2 + 2 D-glucose | - |
? | |
| 3.2.1.207 | Glc2Man9GlcNAc2 + H2O | - |
Sus scrofa | Man9GlcNAc2 + alpha-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.207 | tetramer | 4 * 100000, SDS-PAGE | Sus scrofa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 6 | 7.5 | - |
Sus scrofa |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.207 | 6 | - |
inactivation below | Sus scrofa |
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