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Literature summary extracted from

  • Miasnikov, A.N.
    Characterization of a novel endo-levanase and its gene from Bacillus sp. L7 (1997), FEMS Microbiol. Lett., 154, 23-28.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.65 expression in B. subtilis Bacillus sp. L7

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.65 Ag+
-
 Bacillus sp. L7
3.2.1.65 Ca2+
-
 Bacillus sp. L7
3.2.1.65 Cu2+
-
 Bacillus sp. L7
3.2.1.65 Fe3+
-
 Bacillus sp. L7
3.2.1.65 Hg2+
-
 Bacillus sp. L7
3.2.1.65 Mg2+
-
 Bacillus sp. L7
3.2.1.65 Mn2+
-
 Bacillus sp. L7
3.2.1.65 Na+
-
 Bacillus sp. L7
3.2.1.65 sucrose weak inhibitory effect at high concentrations Bacillus sp. L7
3.2.1.65 Zn2+
-
 Bacillus sp. L7

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.65 extracellular
-
 Bacillus sp. L7
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.65 86000
-
 SDS-PAGE and DNA sequence analysis Bacillus sp. L7

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.65 Bacillus sp. L7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.65
-
 Bacillus sp. L7

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.65 128
-
 U/mg Bacillus sp. L7

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.65 levan + H2O
-
 Bacillus sp. L7 fructose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.65 50
-
-
 Bacillus sp. L7

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.65 5.5
-
 no activity loss at pH 6.0 and 60°C for 20 min Bacillus sp. L7