Literature summary extracted from

Martin, I.; De Barbouille, M.; Ferrari, E.; Klier, A.; Rapoport, G.
Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase (1987), Mol. Gen. Genet., 208, 177-184.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.65	expression in Escherichia coli	Bacillus subtilis

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.2.1.65	extracellular	-	Bacillus subtilis	-	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.2.1.65	73000	-	SDS-PAGE	Bacillus subtilis
3.2.1.65	75870	-	DNA sequence analysis	Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.2.1.65	levans + H2O	Bacillus subtilis	-	oligofructosides	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.65	Bacillus subtilis	-	-	-
3.2.1.65	Escherichia coli	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.65	expression in Escherichia coli	Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.2.1.65	0.3	-	BG4103-strain	Bacillus subtilis
3.2.1.65	0.4	-	QB167-strain	Bacillus subtilis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.65	inulin + H2O	-	Bacillus subtilis	?	-	?
3.2.1.65	levan + H2O	-	Bacillus subtilis	oligofructosides	-	?
3.2.1.65	levans + H2O	-	Bacillus subtilis	oligofructosides	-	?
3.2.1.65	sucrose + H2O	-	Bacillus subtilis	D-fructose + D-glucose	-	?

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Release 2023.1 (January 2023)