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Literature summary extracted from

  • Okada, G.; Takayanagi, T.; Miyahara, S.; Sawai, T.
    An isomalto-dextranase accompanied by isopullulanase activity from Arthrobacter globiformis T6 (1988), Agric. Biol. Chem., 52, 829-836.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.57 Ag+ 5 mM, partial inhibition Arthrobacter globiformis
3.2.1.57 Fe3+ 5 mM completeinactivation Arthrobacter globiformis
3.2.1.57 Hg2+ 5 mM complete inactivation Arthrobacter globiformis
3.2.1.57 KMnO4 5 mM complete inactivation Arthrobacter globiformis
3.2.1.57 N-bromosuccinimide 5 mM complete inactivation Arthrobacter globiformis
3.2.1.94 Ag+
-
 Arthrobacter globiformis
3.2.1.94 Fe3+
-
 Arthrobacter globiformis
3.2.1.94 Hg2+
-
 Arthrobacter globiformis
3.2.1.94 KMnO4
-
 Arthrobacter globiformis
3.2.1.94 N-bromosuccinimide
-
 Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.57 pullulan + H2O Arthrobacter globiformis
-
 isopanose + (Glc)6
-
 ?
3.2.1.57 pullulan + H2O Arthrobacter globiformis T6
-
 isopanose + (Glc)6
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.57 Arthrobacter globiformis
-
 T6, single enzyme with G2-dextranase EC 3.2.1.94 and isopullanase activity
-
3.2.1.94 Arthrobacter globiformis
-
 T6
-
3.2.1.94 Arthrobacter globiformis T6
-
 T6
-

Oxidation Stability

EC Number Oxidation Stability Organism
3.2.1.57 photooxidation in presence of Rose Bengal Arthrobacter globiformis
3.2.1.94 photooxidation with Rose Bengal Arthrobacter globiformis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.57
-
 Arthrobacter globiformis
3.2.1.94
-
 Arthrobacter globiformis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.94 culture filtrate
-
 Arthrobacter globiformis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.94 18.8
-
-
 Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.57 pullulan + H2O
-
 Arthrobacter globiformis isopanose + (Glc)6
-
 ?
3.2.1.57 pullulan + H2O
-
 Arthrobacter globiformis T6 isopanose + (Glc)6
-
 ?
3.2.1.94 pullulan + H2O
-
 Arthrobacter globiformis isopanose
-
 ?
3.2.1.94 pullulan + H2O
-
 Arthrobacter globiformis T6 isopanose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.94 G2-dextranase
-
 Arthrobacter globiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.57 60
-
-
 Arthrobacter globiformis
3.2.1.94 65
-
-
 Arthrobacter globiformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.57 50
-
 below completely stable Arthrobacter globiformis
3.2.1.57 70
-
 10 min, 80% loss of activity Arthrobacter globiformis
3.2.1.57 80
-
 inactivated Arthrobacter globiformis
3.2.1.94 50
-
 stable below 50 Arthrobacter globiformis
3.2.1.94 70
-
 20% activity after 10 min Arthrobacter globiformis
3.2.1.94 80
-
 complete inactivation after 10 min Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.57 4.8
-
-
 Arthrobacter globiformis
3.2.1.94 5.3
-
-
 Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.57 2.8 8 4°C Arthrobacter globiformis
3.2.1.57 4.1 8 45°C Arthrobacter globiformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.94 2.8 8 4°C, 24 h Arthrobacter globiformis
3.2.1.94 4.1 8 45°C, 2 h Arthrobacter globiformis