Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Izumi, T.; Suzuki, K.
    Neutral beta-N-acetylhexosaminidases of rat brain. Purification and enzymatic and immunological characterization (1983), J. Biol. Chem., 258, 6991-6999.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 bovine serum albumin N-acetylgalactosaminidase, stimulation up to 4fold, stimulation of GM2-hydrolysis, effect on N-acetylglucosaminidase only slight Rattus norvegicus
3.2.1.52 dithiothreitol stimulates N-acetylglucosaminidase even at low concentrations Rattus norvegicus
3.2.1.52 neutral N-acetylglucosaminidase stimulates GM2-hydrolysis by N-acetylgalactosaminidase Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 N-acetyl-D-galactosamine inhibits N-galactosaminidase Rattus norvegicus
3.2.1.52 N-acetyl-D-glucosamine inhibits N-glucosaminidase Rattus norvegicus
3.2.1.53 N-acetylgalactosamine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.42
-
 4-methylumbelliferyl beta-N-acetylgalactosaminide N-acetylgalactosaminidase Rattus norvegicus
3.2.1.52 0.45
-
 4-methylumbelliferyl beta-N-acetylgalactosaminide N-acetylglucosaminidase Rattus norvegicus
3.2.1.53 0.42
-
 4-methylumbelliferyl beta-N-acetylgalactosaminide
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.53 lysosome
-
 Rattus norvegicus 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 55000
-
 neutral N-acetylgalactosaminidase, SDS-PAGE Rattus norvegicus
3.2.1.52 82000
-
 neutral N-acetylgalactosaminidase, sucrose density gradient centrifugation Rattus norvegicus
3.2.1.52 88000
-
 neutral N-acetylglucosaminidase, sucrose density gradient centrifugation Rattus norvegicus
3.2.1.52 130000
-
 neutral N-acetylglucosaminidase, SDS-PAGE Rattus norvegicus
3.2.1.52 210000
-
 neutral N-acetylgalactosaminidase, gel filtration Rattus norvegicus
3.2.1.52 525000
-
 neutral N-acetylglucosaminidase, gel filtration Rattus norvegicus
3.2.1.53 82000
-
 sucrose density gradient centrifugation Rattus norvegicus
3.2.1.53 210000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Rattus norvegicus
-
 N-acetyl-D-hexosamines
-
 ?
3.2.1.53 N-acetyl-beta-D-galactosaminides + H2O Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Rattus norvegicus
-
-
-
3.2.1.53 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 N-acetylhexosaminidase: 63fold Rattus norvegicus
3.2.1.52 neutral N-acetylgalactosaminidase, 2000-2500fold to homogeneity Rattus norvegicus
3.2.1.52 neutral N-acetylglucosaminidase, 300-330fold, 85-90% pure Rattus norvegicus
3.2.1.53
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 brain
-
 Rattus norvegicus
-
3.2.1.53 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.53 9.8
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl beta-N-acetylgalactosaminide + H2O
-
 Rattus norvegicus 4-methylumbelliferone + beta-N-acetylgalactosamine
-
 ?
3.2.1.52 4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-galactopyranoside + H2O
-
 Rattus norvegicus 4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-galactopyranoside
-
 ?
3.2.1.52 asialo GM2 ganglioside + H2O neutral N-acetylgalactosaminidase Rattus norvegicus lactosylceramide
-
 ?
3.2.1.52 globoside + H2O neutral N-acetylgalactosaminidase Rattus norvegicus ?
-
 ?
3.2.1.52 GM2 ganglioside + H2O neutral N-acetylgalactosaminidase Rattus norvegicus GM3 ganglioside + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Rattus norvegicus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O hydrolyzed by neutral N-acetylgalactosaminidase and to a very small extend by neutral N-acetylglucosaminidase Rattus norvegicus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O neutral N-acetylglucosaminidase Rattus norvegicus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.53 4-methylumbelliferyl beta-N-acetylgalactosaminide + H2O
-
 Rattus norvegicus 4-methylumbelliferone + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.53 N-acetyl-beta-D-galactosaminides + H2O e.g. 4-methylumbelliferyl-beta-N-acetylgalactosaminide Rattus norvegicus N-acetyl-D-galactosamine + an alcohol e.g. 4-methylumbelliferone ?
3.2.1.53 N-acetyl-beta-D-galactosaminides + H2O
-
 Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 50
-
 N-glucosaminidase: complete loss of activity after 30 min with or without addition of bovine serum albumin, N-galactosaminidase: loss of 20% of activity after 30 min with bovine serum albumin Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5.2
-
 substrate: 4-methylumbelliferyl-beta-N-acetylgalactosaminide, N-acetylglucosaminidase Rattus norvegicus
3.2.1.52 6
-
 substrate: 4-methylumbelliferyl-beta-N-acetylgalactosaminide, N-acetylgalactosaminidase Rattus norvegicus
3.2.1.53 6
-
 4-methylumbelliferyl beta-N-acetylgalactosaminide Rattus norvegicus