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Literature summary extracted from

  • Legler, G.; Liedtke, H.
    Glucosylceramidase from calf spleen. Characterization of its active site with 4-n-alkylumbelliferyl beta-glucosides and N-alkyl derivatives of 1-deoxynojirimycin (1985), Biol. Chem. Hoppe-Seyler, 366, 1113-1122.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.45 1-deoxynojirimycin
-
 Bos taurus
3.2.1.45 N-butyl-1-deoxynojirimycin
-
 Bos taurus
3.2.1.45 N-decyl-1-deoxynojirimycin
-
 Bos taurus
3.2.1.45 N-dodecyl-1-deoxynojirimycin
-
 Bos taurus
3.2.1.45 N-hexyl-1-deoxynojirimycin
-
 Bos taurus
3.2.1.45 N-methyl-1-deoxynojirimycin
-
 Bos taurus
3.2.1.45 N-propyl-1-deoxynojirimycin
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.45 0.026
-
 4-nonylumbelliferyl-beta-D-glucopyranoside in presence of taurocholate Bos taurus
3.2.1.45 0.038
-
 4-undecylumbelliferyl beta-D-glucopyranoside in presence of taurocholate Bos taurus
3.2.1.45 0.045
-
 4-nonylumbelliferyl-beta-D-glucopyranoside
-
 Bos taurus
3.2.1.45 3.6
-
 4-methylumbelliferyl-beta-D-glucopyranoside
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.45 glucocerebroside + H2O Bos taurus
-
 glucose + ceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.45 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.45
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.45 spleen
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.45 4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Bos taurus methylumbelliferone + glucose
-
 ?
3.2.1.45 4-undecylumbelliferyl beta-D-glucopyranoside + H2O
-
 Bos taurus 4-undecylumbelliferone + D-glucose
-
 ?
3.2.1.45 glucocerebroside + H2O
-
 Bos taurus glucose + ceramide
-
 ?
3.2.1.45 heptylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Bos taurus heptylumbelliferone + glucose
-
 ?
3.2.1.45 nonylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Bos taurus nonylumbelliferone + glucose
-
 ?
3.2.1.45 undecylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Bos taurus undecylumbelliferone + glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.45 25
-
 assay at Bos taurus