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Literature summary extracted from
- Guanosine diphosphate D-glucose glucohydrolase (1966), Arch. Biochem. Biophys., 116, 26-33.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.42 | GDP | competitive | Saccharomyces cerevisiae |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.42 | 0.23 | - |
GDPglucose | - |
Saccharomyces cerevisiae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.42 | cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.42 | Mg2+ | relieves inhibition by guanosine diphosphate, some activation | Saccharomyces cerevisiae | |
| 3.2.1.42 | Mn2+ | relieves inhibition by guanosine diphosphate, some activation | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.42 | GDP-glucose + H2O | Saccharomyces cerevisiae | control of intracellular concentration | GDP + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.42 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.42 | - |
Saccharomyces cerevisiae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.42 | additional information | starch-free commercial baker's yeast | Saccharomyces cerevisiae | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.42 | 0.208 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.42 | -20°C, 50% loss of activity after 6 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.42 | GDP-glucose + H2O | strictly specific for | Saccharomyces cerevisiae | GDP + D-glucose | - |
? | |
| 3.2.1.42 | GDP-glucose + H2O | control of intracellular concentration | Saccharomyces cerevisiae | GDP + D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.42 | 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.42 | 5 | 7 | - |
Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.42 | 5 | 9 | - |
Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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