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Literature summary extracted from

  • Koch, R.; Canganella, F.; Hippe, H.; Jahnke, K.D.; Antranikian, G.
    Purification and properties of a thermostable pullulanase from a newly isolated thermophilic anaerobic bacterium Fervidobacterium pennavorans Ven5 (1997), Appl. Environ. Microbiol., 63, 1088-1094.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 extracellular
-
 Fervidobacterium pennivorans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 240000
-
 gel filtration, SDS-PAGE Fervidobacterium pennivorans
3.2.1.41 776000
-
 3 * 776000, SDS-PAGE Fervidobacterium pennivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Fervidobacterium pennivorans branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O Fervidobacterium pennivorans Ven5 branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Fervidobacterium pennivorans
-
 maltotriose + ?
-
 ?
3.2.1.41 pullulan + H2O Fervidobacterium pennivorans Ven5
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Fervidobacterium pennivorans
-
 Ven5
-
3.2.1.41 Fervidobacterium pennivorans Ven5
-
 Ven5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 to homogeneity Fervidobacterium pennivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O
-
 Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylose + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylose + H2O
-
 Fervidobacterium pennivorans Ven5 maltooligosaccharides + maltose
-
 ?
3.2.1.41 dextrin + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 glycogen + H2O
-
 Fervidobacterium pennivorans maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Fervidobacterium pennivorans maltotriose + ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Fervidobacterium pennivorans Ven5 maltotriose + ?
-
 ?
3.2.1.41 soluble starch
-
 Fervidobacterium pennivorans maltotriose + maltose
-
 ?
3.2.1.41 soluble starch
-
 Fervidobacterium pennivorans Ven5 maltotriose + maltose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 trimer 3 * 776000, SDS-PAGE Fervidobacterium pennivorans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type I hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan Fervidobacterium pennivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 85
-
-
 Fervidobacterium pennivorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.41 70 90
-
 Fervidobacterium pennivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 70
-
 after 1 h, no loss of activity Fervidobacterium pennivorans
3.2.1.41 80
-
 pH 6, after 2 h, 50% loss of activity Fervidobacterium pennivorans
3.2.1.41 90
-
 pH 6, after 5 min, 50% loss of activity Fervidobacterium pennivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Fervidobacterium pennivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.41 5 8
-
 Fervidobacterium pennivorans