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Literature summary extracted from

  • Kim, C.H.; Nashiru, O.; Ko, J.H.
    Purification and biochemical characterization of pullulanase type I from Thermus caldophilus GK-24 (1996), FEMS Microbiol. Lett., 138, 147-152.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Cu2+
-
 Thermus caldophilus
3.2.1.41 Cyclodextrins
-
 Thermus caldophilus
3.2.1.41 Fe2+
-
 Thermus caldophilus
3.2.1.41 Zn2+
-
 Thermus caldophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.41 Ca2+
-
 Thermus caldophilus
3.2.1.41 Mg2+
-
 Thermus caldophilus
3.2.1.41 Mn2+
-
 Thermus caldophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 65000
-
 gel filtration, SDS-PAGE Thermus caldophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Thermus caldophilus branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Thermus caldophilus
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Thermus caldophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 to homogeneity Thermus caldophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Thermus caldophilus maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Thermus caldophilus maltooligosaccharides + maltose
-
 ?
3.2.1.41 dextrin + H2O
-
 Thermus caldophilus maltooligosaccharides + maltose
-
 ?
3.2.1.41 glycogen + H2O
-
 Thermus caldophilus maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Thermus caldophilus maltotriose + ?
-
 ?
3.2.1.41 soluble starch
-
 Thermus caldophilus maltotriose + maltose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 monomer 1 * 65000 Thermus caldophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type I hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan Thermus caldophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 75
-
-
 Thermus caldophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.41 95
-
 after 30 min, pH 5.5, no loss of activity Thermus caldophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 5.5
-
-
 Thermus caldophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.41 3.5 8 stable at 4°C for 24 h Thermus caldophilus