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Literature summary extracted from

  • Brandt, C.J.; Catley, B.J.; Awad jr., W.M.
    Extracellular and protease-released pullulanases (1976), J. Bacteriol., 125, 501-508.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.41 Cyclodextrins
-
 Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 0.021 0.078 pullulan glucose equivalents Klebsiella aerogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.41 cytoplasm protease-released Klebsiella aerogenes 5737
-
3.2.1.41 extracellular
-
 Klebsiella aerogenes
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 126000
-
 protease-released enzyme, gel filtration Klebsiella aerogenes
3.2.1.41 141000
-
 extracellular enzyme, gel filtration Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.41 amylopectin + H2O Klebsiella aerogenes branched polysaccharides maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O Klebsiella aerogenes
-
 maltotriose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Klebsiella aerogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 to homogeneity Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
3.2.1.41 4 months, 4°C Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 amylopectin + H2O
-
 Klebsiella aerogenes maltooligosaccharides + maltose
-
 ?
3.2.1.41 amylopectin + H2O branched polysaccharides Klebsiella aerogenes maltooligosaccharides + maltose
-
 ?
3.2.1.41 pullulan + H2O
-
 Klebsiella aerogenes maltotriose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 monomer 1 * 141000, gel electrophoresis Klebsiella aerogenes

Synonyms

EC Number Synonyms Comment Organism
3.2.1.41 pullulanase type II hydrolyzes (1-6)-alpha-D-glucosidic linkages in pullulan and (1-4)-alpha-linkages in other polysaccharides Klebsiella aerogenes