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Literature summary extracted from

  • Balance, G.M.; Manners, D.J.
    Partial purification and properties of an endo-1,3-beta-D-glucanase from germinated rye (1978), Phytochemistry, 17, 1539-1543.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.39 bovine serum albumin marked stimulation dependent on buffer concentration, especially at low concentration Secale cereale

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 additional information
-
 Laminarin value in mg/ml Secale cereale
3.2.1.39 additional information
-
 Laminarin 0.25 Secale cereale

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.39 Ca2+ nearly 3fold activation at 5 mM Secale cereale
3.2.1.39 Mg2+ 3.5fold activation at 5 mM Secale cereale
3.2.1.39 Na+ slight activation at 5 mM Secale cereale

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 24300
-
 gel filtration Secale cereale

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Secale cereale
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 partial Secale cereale

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 seed germinating grain Secale cereale
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc + H2O smallest substrate, products are smaller oligosaccharides and glucose Secale cereale ?
-
 ?
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc + H2O laminaritriose Secale cereale ?
-
 ?
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O products are smaller oligosaccharides and glucose Secale cereale ?
-
 ?
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O laminaritetraose Secale cereale ?
-
 ?
3.2.1.39 laminarin + H2O preferred substrate Secale cereale D-glucose + ?
-
 ?
3.2.1.39 O-carboxymethyl-pachyman + H2O preferred substrate Secale cereale ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 45
-
-
 Secale cereale

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 45
-
 maximum heat stability Secale cereale

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5
-
 hydrolysis of laminarin Secale cereale
3.2.1.39 5.7
-
 hydrolysis of CM-pachyman Secale cereale