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Literature summary extracted from

  • Nogi, Y.; Horikoshi, K.
    A thermostable alkaline beta-1,3-glucanase produced by alkalophilic Bacillus sp. AG-430 (1990), Appl. Microbiol. Biotechnol., 32, 704-707.
No PubMed abstract available

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.39 extracellular
-
 Bacillus sp. (in: Bacteria)
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 35000
-
 SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Bacillus sp. (in: Bacteria)
-
 enzyme synthesis induced in pachyman-containing medium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 to homogeneity, 3step chromatography Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 culture filtrate
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 369.6
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O end-products are laminaritriose, laminaribiose and glucose, exhibits weak glucosyltransferase activity Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.39 3-O-beta-D-Glc-D-Glc-D-Glc-D-Glc + H2O laminaritetraose Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.39 laminarin + H2O major products are glucose, laminaribiose, laminaritriose, laminaritetraose and higher liminarioligosaccharides Bacillus sp. (in: Bacteria) D-glucose + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 60 65
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 75
-
 50% activity retained Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 9 10
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.39 7 8 most stable within Bacillus sp. (in: Bacteria)