BRENDA - Enzyme Database

Purification and some properties of beta-xylosidase from Eimericella nidulans

Matsuo, M.; Yasui, T.; Agric. Biol. Chem. 48, 1853-1860 (1984)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
Hg2+
strong inhibition at 1 mM
Aspergillus nidulans
3.2.1.37
N-bromosuccinimide
complete inhibition at 1 mM
Aspergillus nidulans
3.2.1.37
p-chloromercuribenzoate
strong inhibition
Aspergillus nidulans
3.2.1.37
SDS
strong inhibition at 1 mM
Aspergillus nidulans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.37
extracellular
-
Aspergillus nidulans
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.37
116000
-
2 * 116000, SDS-PAGE
Aspergillus nidulans
3.2.1.37
240000
-
gel filtration, SDS-PAGE
Aspergillus nidulans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.37
xylobiose + H2O
Aspergillus nidulans
-
beta-D-xylose + D-xylose
-
Aspergillus nidulans
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.37
Aspergillus nidulans
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.37
glycoprotein
4% carbohydrate moiety
Aspergillus nidulans
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.37
to homogeneity by chromatography, electrofocusing
Aspergillus nidulans
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.37
cell culture
-
Aspergillus nidulans
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.37
62.9
-
-
Aspergillus nidulans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.37
o-chlorophenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
o-chlorophenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
o-methoxyphenyl beta-D-xyloside
-
136305
Aspergillus nidulans
o-methoxyphenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
o-methylphenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
o-methylphenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
o-nitrophenyl-beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
o-nitrophenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
p-chlorophenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
p-chlorophenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
p-methoxyphenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
p-methoxyphenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
p-methylphenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
p-methylphenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
p-nitrophenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
p-nitrophenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
phenyl beta-D-xyloside + H2O
with retention of configuration
136305
Aspergillus nidulans
phenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
xylobiose + H2O
-
136305
Aspergillus nidulans
beta-D-xylose + D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
xylopentaose + H2O
-
136305
Aspergillus nidulans
beta-D-xylose + ?
-
136305
Aspergillus nidulans
?
3.2.1.37
xylotetraose + H2O
-
136305
Aspergillus nidulans
beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
xylotriose + H2O
-
136305
Aspergillus nidulans
beta-D-xylose + xylobiose
-
136305
Aspergillus nidulans
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.37
dimer
2 * 116000, SDS-PAGE
Aspergillus nidulans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
55
-
-
Aspergillus nidulans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
50
-
stable up to
Aspergillus nidulans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
4.5
5
-
Aspergillus nidulans
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.37
4.5
5
stable for 30 min at 50°C
Aspergillus nidulans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
Hg2+
strong inhibition at 1 mM
Aspergillus nidulans
3.2.1.37
N-bromosuccinimide
complete inhibition at 1 mM
Aspergillus nidulans
3.2.1.37
p-chloromercuribenzoate
strong inhibition
Aspergillus nidulans
3.2.1.37
SDS
strong inhibition at 1 mM
Aspergillus nidulans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.37
extracellular
-
Aspergillus nidulans
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.37
116000
-
2 * 116000, SDS-PAGE
Aspergillus nidulans
3.2.1.37
240000
-
gel filtration, SDS-PAGE
Aspergillus nidulans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.37
xylobiose + H2O
Aspergillus nidulans
-
beta-D-xylose + D-xylose
-
Aspergillus nidulans
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.37
glycoprotein
4% carbohydrate moiety
Aspergillus nidulans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.37
to homogeneity by chromatography, electrofocusing
Aspergillus nidulans
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.37
cell culture
-
Aspergillus nidulans
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.37
62.9
-
-
Aspergillus nidulans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.37
o-chlorophenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
o-chlorophenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
o-methoxyphenyl beta-D-xyloside
-
136305
Aspergillus nidulans
o-methoxyphenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
o-methylphenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
o-methylphenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
o-nitrophenyl-beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
o-nitrophenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
p-chlorophenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
p-chlorophenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
p-methoxyphenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
p-methoxyphenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
p-methylphenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
p-methylphenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
p-nitrophenyl beta-D-xyloside + H2O
-
136305
Aspergillus nidulans
p-nitrophenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
phenyl beta-D-xyloside + H2O
with retention of configuration
136305
Aspergillus nidulans
phenol + beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
xylobiose + H2O
-
136305
Aspergillus nidulans
beta-D-xylose + D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
xylopentaose + H2O
-
136305
Aspergillus nidulans
beta-D-xylose + ?
-
136305
Aspergillus nidulans
?
3.2.1.37
xylotetraose + H2O
-
136305
Aspergillus nidulans
beta-D-xylose
-
136305
Aspergillus nidulans
?
3.2.1.37
xylotriose + H2O
-
136305
Aspergillus nidulans
beta-D-xylose + xylobiose
-
136305
Aspergillus nidulans
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.37
dimer
2 * 116000, SDS-PAGE
Aspergillus nidulans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
55
-
-
Aspergillus nidulans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
50
-
stable up to
Aspergillus nidulans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
4.5
5
-
Aspergillus nidulans
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.37
4.5
5
stable for 30 min at 50°C
Aspergillus nidulans