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Literature summary extracted from

  • Shao, W.; Wiegel, J.
    Purification and characterization of a thermostable beta-xylosidase from Thermoanaerobacter ethanolicus (1992), J. Bacteriol., 174, 5848-5853.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 o-nitrophenyl beta-D-xylopyranoside substrate inhibition if concentration higher than 0.5 mM Thermoanaerobacter ethanolicus
3.2.1.37 p-nitrophenyl alpha-L-arabinofuranoside substrate inhibition if concentration higher than 12 mM Thermoanaerobacter ethanolicus
3.2.1.37 p-nitrophenyl alpha-L-arabinopyranoside substrate inhibition if concentration higher than 9 mM Thermoanaerobacter ethanolicus
3.2.1.37 p-nitrophenyl beta-D-xylopyranoside substrate inhibition if concentration higher than 0.1-0.2 mM Thermoanaerobacter ethanolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.018
-
 p-nitrophenyl beta-D-xylopyranoside at pH 5.0-5.2 Thermoanaerobacter ethanolicus
3.2.1.37 0.038
-
 p-nitrophenyl beta-D-xylopyranoside at pH 5.8-6.0 Thermoanaerobacter ethanolicus
3.2.1.37 0.06
-
 o-nitrophenyl beta-D-xylopyranoside
-
 Thermoanaerobacter ethanolicus
3.2.1.37 2.1
-
 p-nitrophenyl alpha-L-arabinopyranoside
-
 Thermoanaerobacter ethanolicus
3.2.1.37 4.6
-
 p-nitrophenyl alpha-L-arabinofuranoside
-
 Thermoanaerobacter ethanolicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.37 extracellular
-
 Thermoanaerobacter ethanolicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 85000
-
 2 * 85000, SDS-PAGE, native PAGE Thermoanaerobacter ethanolicus
3.2.1.37 165000
-
 native PAGE, gel filtration, SDS-PAGE Thermoanaerobacter ethanolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 xylobiose + H2O Thermoanaerobacter ethanolicus
-
 beta-D-xylose + D-xylose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Thermoanaerobacter ethanolicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 to homogeneity by 3step chromatography techniques Thermoanaerobacter ethanolicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 cell culture highest enzyme synthesis in sucrose/xylose medium Thermoanaerobacter ethanolicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 122
-
 for p-nitrophenyl beta-D-xylopyranoside Thermoanaerobacter ethanolicus
3.2.1.37 183
-
 for o-nitrophenyl beta-D-xylopyranoside Thermoanaerobacter ethanolicus
3.2.1.37 758
-
 for p-nitrophenyl alpha-L-arabinopyranoside Thermoanaerobacter ethanolicus
3.2.1.37 1073
-
 for p-nitrophenyl alpha-L-arabinofuranoside Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 o-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermoanaerobacter ethanolicus o-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 p-nitrophenyl alpha-L-arabinofuranoside + H2O highest activity Thermoanaerobacter ethanolicus p-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 p-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Thermoanaerobacter ethanolicus p-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermoanaerobacter ethanolicus p-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.37 xylobiose + H2O
-
 Thermoanaerobacter ethanolicus beta-D-xylose + D-xylose
-
 ?
3.2.1.37 xylopentaose + H2O lower activity than on xylobiose Thermoanaerobacter ethanolicus beta-D-xylose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 dimer 2 * 85000, SDS-PAGE, native PAGE Thermoanaerobacter ethanolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 75 80 hydrolysis of p-nitrophenyl alpha-L-arabinofuranoside and p-nitrophenyl alpha-L-arabinopyranoside Thermoanaerobacter ethanolicus
3.2.1.37 82
-
 at optimal pH 5.0-5.2 and hydrolysis of p-nitrophenyl beta-D-xylopyranoside Thermoanaerobacter ethanolicus
3.2.1.37 90
-
 hydrolysis of o-nitrophenyl beta-D-xylopyranoside Thermoanaerobacter ethanolicus
3.2.1.37 93
-
 at optimal pH 5.8-6.0 and hydrolysis of p-nitrophenyl beta-D-xylopyranoside Thermoanaerobacter ethanolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 75
-
 stable for more than 1 h, half-life at 86°C for 15 min Thermoanaerobacter ethanolicus
3.2.1.37 86
-
 half-life 15 min Thermoanaerobacter ethanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5 5.2 at optimal temperature of 82°C and hydrolysis of p-nitrophenyl beta-D-xylopyranoside Thermoanaerobacter ethanolicus
3.2.1.37 5.8 6 at optimal temperature of 93°C and hydrolysis of p-nitrophenyl beta-D-xylopyranoside Thermoanaerobacter ethanolicus
3.2.1.37 6
-
 hydrolysis of p-nitrophenyl alpha-L-arabinofuranoside, p-nitrophenyl alpha-L-arabinopyranoside, o-nitrophenyl beta-D-xylopyranoside Thermoanaerobacter ethanolicus