Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hirata, K.; Nakahara, Y.; Kimura, Y.; Funatsu, G.
    Purification and some properties of a beta-xylosidase and an alpha-fucosidase from apple snails (Pomacea canaliculata) (1996), Biosci. Biotechnol. Biochem., 60, 249-254.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 p-chloromercuribenzoate 29% inhibition at 1 mM Pomacea canaliculata
3.2.1.51 4-chloromercuribenzoate 71% inhibition at 1 mM Pomacea canaliculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 85000 96000 SDS-PAGE, sedimentation coefficient Pomacea canaliculata
3.2.1.51 54000
-
 4 * 54000, SDS-PAGE Pomacea canaliculata
3.2.1.51 230000
-
 sedimentation equilibrium centrifugation Pomacea canaliculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.37 beta-1,3-xylan + H2O Pomacea canaliculata complete hydrolysis after prolonged incubation beta-D-xylose + ?
-
 ?
3.2.1.37 beta-1,4-xylan + H2O Pomacea canaliculata complete hydrolysis after prolonged incubation beta-D-xylose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Pomacea canaliculata
-
 apple snail
-
3.2.1.51 Pomacea canaliculata
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein 4.6% sugar content Pomacea canaliculata
3.2.1.51 glycoprotein
-
 Pomacea canaliculata

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 to homogeneity by 4step chromatography techniques Pomacea canaliculata
3.2.1.51 to homogeneity, chromatography steps Pomacea canaliculata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 viscus
-
 Pomacea canaliculata
-
3.2.1.51 viscus
-
 Pomacea canaliculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 0.95
-
-
 Pomacea canaliculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 beta-1,3-xylan + H2O complete hydrolysis after prolonged incubation Pomacea canaliculata beta-D-xylose + ?
-
 ?
3.2.1.37 beta-1,4-xylan + H2O complete hydrolysis after prolonged incubation Pomacea canaliculata beta-D-xylose + ?
-
 ?
3.2.1.37 Manalpha(1-6)[Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc--pyridylaminoside + H2O complete hydrolysis Pomacea canaliculata beta-D-xylose + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
 ?
3.2.1.37 p-nitrophenyl beta-D-xyloside + H2O
-
 Pomacea canaliculata p-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 Xylbeta(1-2)Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O complete hydrolysis Pomacea canaliculata beta-D-xylose + Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
 ?
3.2.1.37 [Manalpha(1-6)Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O 50% hydrolysis Pomacea canaliculata beta-D-xylose + [Manalpha(1-6)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
 ?
3.2.1.51 4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
 Pomacea canaliculata 4-nitrophenol + alpha-L-fucose
-
 ?
3.2.1.51 GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues Pomacea canaliculata alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
 ?
3.2.1.51 Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
-
 Pomacea canaliculata alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
 ?
3.2.1.51 Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
-
 Pomacea canaliculata alpha-L-fucose + Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-pyridylaminoside
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 monomer 1 * 85000-96000, SDS-PAGE, sedimentation coefficient Pomacea canaliculata
3.2.1.51 tetramer 4 * 54000, SDS-PAGE Pomacea canaliculata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 60
-
 stable up to within 3 min Pomacea canaliculata
3.2.1.51 65
-
 fairly stable up to Pomacea canaliculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 3.6
-
-
 Pomacea canaliculata
3.2.1.51 3.2 5.2
-
 Pomacea canaliculata
3.2.1.51 5.7
-
-
 Pomacea canaliculata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 4 10 unstable below pH 3, activity lost at pH 2 Pomacea canaliculata
3.2.1.51 4 10 fairly stable within Pomacea canaliculata