BRENDA - Enzyme Database

Purification and some properties of a beta-xylosidase and an alpha-fucosidase from apple snails (Pomacea canaliculata)

Hirata, K.; Nakahara, Y.; Kimura, Y.; Funatsu, G.; Biosci. Biotechnol. Biochem. 60, 249-254 (1996)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
p-chloromercuribenzoate
29% inhibition at 1 mM
Pomacea canaliculata
3.2.1.51
4-chloromercuribenzoate
71% inhibition at 1 mM
Pomacea canaliculata
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.37
85000
96000
SDS-PAGE, sedimentation coefficient
Pomacea canaliculata
3.2.1.51
54000
-
4 * 54000, SDS-PAGE
Pomacea canaliculata
3.2.1.51
230000
-
sedimentation equilibrium centrifugation
Pomacea canaliculata
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.37
beta-1,3-xylan + H2O
Pomacea canaliculata
complete hydrolysis after prolonged incubation
beta-D-xylose + ?
-
Pomacea canaliculata
?
3.2.1.37
beta-1,4-xylan + H2O
Pomacea canaliculata
complete hydrolysis after prolonged incubation
beta-D-xylose + ?
-
Pomacea canaliculata
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.37
Pomacea canaliculata
-
apple snail
-
3.2.1.51
Pomacea canaliculata
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.37
glycoprotein
4.6% sugar content
Pomacea canaliculata
3.2.1.51
glycoprotein
-
Pomacea canaliculata
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.37
to homogeneity by 4step chromatography techniques
Pomacea canaliculata
3.2.1.51
to homogeneity, chromatography steps
Pomacea canaliculata
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.37
viscus
-
Pomacea canaliculata
-
3.2.1.51
viscus
-
Pomacea canaliculata
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.37
0.95
-
-
Pomacea canaliculata
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.37
beta-1,3-xylan + H2O
complete hydrolysis after prolonged incubation
136278
Pomacea canaliculata
beta-D-xylose + ?
-
136278
Pomacea canaliculata
?
3.2.1.37
beta-1,4-xylan + H2O
complete hydrolysis after prolonged incubation
136278
Pomacea canaliculata
beta-D-xylose + ?
-
136278
Pomacea canaliculata
?
3.2.1.37
Manalpha(1-6)[Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc--pyridylaminoside + H2O
complete hydrolysis
136278
Pomacea canaliculata
beta-D-xylose + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.37
p-nitrophenyl beta-D-xyloside + H2O
-
136278
Pomacea canaliculata
p-nitrophenol + beta-D-xylose
-
136278
Pomacea canaliculata
?
3.2.1.37
Xylbeta(1-2)Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O
complete hydrolysis
136278
Pomacea canaliculata
beta-D-xylose + Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.37
[Manalpha(1-6)Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O
50% hydrolysis
136278
Pomacea canaliculata
beta-D-xylose + [Manalpha(1-6)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
136278
Pomacea canaliculata
4-nitrophenol + alpha-L-fucose
-
136278
Pomacea canaliculata
?
3.2.1.51
GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues
136278
Pomacea canaliculata
alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.51
Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
-
136278
Pomacea canaliculata
alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.51
Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
-
136278
Pomacea canaliculata
alpha-L-fucose + Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-pyridylaminoside
-
136278
Pomacea canaliculata
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.37
monomer
1 * 85000-96000, SDS-PAGE, sedimentation coefficient
Pomacea canaliculata
3.2.1.51
tetramer
4 * 54000, SDS-PAGE
Pomacea canaliculata
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
60
-
stable up to within 3 min
Pomacea canaliculata
3.2.1.51
65
-
fairly stable up to
Pomacea canaliculata
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
3.6
-
-
Pomacea canaliculata
3.2.1.51
3.2
5.2
-
Pomacea canaliculata
3.2.1.51
5.7
-
-
Pomacea canaliculata
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.37
4
10
unstable below pH 3, activity lost at pH 2
Pomacea canaliculata
3.2.1.51
4
10
fairly stable within
Pomacea canaliculata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
p-chloromercuribenzoate
29% inhibition at 1 mM
Pomacea canaliculata
3.2.1.51
4-chloromercuribenzoate
71% inhibition at 1 mM
Pomacea canaliculata
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.37
85000
96000
SDS-PAGE, sedimentation coefficient
Pomacea canaliculata
3.2.1.51
54000
-
4 * 54000, SDS-PAGE
Pomacea canaliculata
3.2.1.51
230000
-
sedimentation equilibrium centrifugation
Pomacea canaliculata
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.2.1.37
beta-1,3-xylan + H2O
Pomacea canaliculata
complete hydrolysis after prolonged incubation
beta-D-xylose + ?
-
Pomacea canaliculata
?
3.2.1.37
beta-1,4-xylan + H2O
Pomacea canaliculata
complete hydrolysis after prolonged incubation
beta-D-xylose + ?
-
Pomacea canaliculata
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.2.1.37
glycoprotein
4.6% sugar content
Pomacea canaliculata
3.2.1.51
glycoprotein
-
Pomacea canaliculata
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.37
to homogeneity by 4step chromatography techniques
Pomacea canaliculata
3.2.1.51
to homogeneity, chromatography steps
Pomacea canaliculata
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.37
viscus
-
Pomacea canaliculata
-
3.2.1.51
viscus
-
Pomacea canaliculata
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.37
0.95
-
-
Pomacea canaliculata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.37
beta-1,3-xylan + H2O
complete hydrolysis after prolonged incubation
136278
Pomacea canaliculata
beta-D-xylose + ?
-
136278
Pomacea canaliculata
?
3.2.1.37
beta-1,4-xylan + H2O
complete hydrolysis after prolonged incubation
136278
Pomacea canaliculata
beta-D-xylose + ?
-
136278
Pomacea canaliculata
?
3.2.1.37
Manalpha(1-6)[Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc--pyridylaminoside + H2O
complete hydrolysis
136278
Pomacea canaliculata
beta-D-xylose + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.37
p-nitrophenyl beta-D-xyloside + H2O
-
136278
Pomacea canaliculata
p-nitrophenol + beta-D-xylose
-
136278
Pomacea canaliculata
?
3.2.1.37
Xylbeta(1-2)Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O
complete hydrolysis
136278
Pomacea canaliculata
beta-D-xylose + Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.37
[Manalpha(1-6)Manalpha(1-3)Xylbeta(1-2)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside + H2O
50% hydrolysis
136278
Pomacea canaliculata
beta-D-xylose + [Manalpha(1-6)Manalpha(1-6)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.51
4-nitrophenyl-alpha-L-fucopyranoside + H2O
-
136278
Pomacea canaliculata
4-nitrophenol + alpha-L-fucose
-
136278
Pomacea canaliculata
?
3.2.1.51
GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues
136278
Pomacea canaliculata
alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.51
Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
-
136278
Pomacea canaliculata
alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
136278
Pomacea canaliculata
?
3.2.1.51
Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
-
136278
Pomacea canaliculata
alpha-L-fucose + Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-pyridylaminoside
-
136278
Pomacea canaliculata
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.37
monomer
1 * 85000-96000, SDS-PAGE, sedimentation coefficient
Pomacea canaliculata
3.2.1.51
tetramer
4 * 54000, SDS-PAGE
Pomacea canaliculata
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
60
-
stable up to within 3 min
Pomacea canaliculata
3.2.1.51
65
-
fairly stable up to
Pomacea canaliculata
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
3.6
-
-
Pomacea canaliculata
3.2.1.51
3.2
5.2
-
Pomacea canaliculata
3.2.1.51
5.7
-
-
Pomacea canaliculata
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.37
4
10
unstable below pH 3, activity lost at pH 2
Pomacea canaliculata
3.2.1.51
4
10
fairly stable within
Pomacea canaliculata