BRENDA - Enzyme Database

Purification and characterization of beta-1,3-xylanase from a marine bacterium Alcaligenes sp. XY-234

Araki, T.; Inoue, N.; Morishita, T.; J. Gen. Appl. Microbiol. 44, 269-274 (1998) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.32
Ag+
1 mM, over 90% inhibition
Alcaligenes sp.
3.2.1.32
Al3+
1 mM AlCl3, strong
Alcaligenes sp.
3.2.1.32
Cu2+
1 mM CuCl2, strong
Alcaligenes sp.
3.2.1.32
Fe3+
1 mM FeCl3, strong
Alcaligenes sp.
3.2.1.32
Hg2+
1 mM, strong
Alcaligenes sp.
3.2.1.32
N-bromosuccinimide
-
Alcaligenes sp.
3.2.1.32
Pb(CH3COO)2
1 mM, strong
Alcaligenes sp.
3.2.1.32
Pb2+
1 mM, over 90% inhibition
Alcaligenes sp.
3.2.1.32
Zn2+
1 mM, over 90% inhibition
Alcaligenes sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.32
additional information
-
additional information
-
Alcaligenes sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.32
59000
-
x * 59000, SDS-PAGE
Alcaligenes sp.
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.32
Alcaligenes sp.
-
XY-234
-
3.2.1.32
Alcaligenes sp. XY-234
-
XY-234
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.32
-
Alcaligenes sp.
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.32
culture medium
-
Alcaligenes sp.
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.32
17.5
-
-
Alcaligenes sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.32
beta-1,3-xylan + H2O
-
136250
Alcaligenes sp.
xylose + xylooligosaccharides
-
136250
Alcaligenes sp.
?
3.2.1.32
beta-1,3-xylan + H2O
-
136250
Alcaligenes sp. XY-234
xylose + xylooligosaccharides
-
136250
Alcaligenes sp. XY-234
?
3.2.1.32
additional information
no hydrolysis of xylobiose and p-nitrophenyl-beta-D-xyloside
136250
Alcaligenes sp.
?
-
-
-
?
3.2.1.32
additional information
no hydrolysis of xylobiose and p-nitrophenyl-beta-D-xyloside
136250
Alcaligenes sp. XY-234
?
-
-
-
?
3.2.1.32
xylopentaose + H2O
-
136250
Alcaligenes sp.
xylose + xylotetraose + xylobiose + xylotriose
-
136250
Alcaligenes sp.
?
3.2.1.32
xylopentaose + H2O
-
136250
Alcaligenes sp. XY-234
xylose + xylotetraose + xylobiose + xylotriose
-
136250
Alcaligenes sp. XY-234
?
3.2.1.32
xylotetraose + H2O
-
136250
Alcaligenes sp.
xylose + xylotriose + xylobiose
-
136250
Alcaligenes sp.
?
3.2.1.32
xylotetraose + H2O
-
136250
Alcaligenes sp. XY-234
xylose + xylotriose + xylobiose
-
136250
Alcaligenes sp. XY-234
?
3.2.1.32
xylotriose + H2O
-
136250
Alcaligenes sp.
xylose + xylobiose
-
136250
Alcaligenes sp.
?
3.2.1.32
xylotriose + H2O
-
136250
Alcaligenes sp. XY-234
xylose + xylobiose
-
136250
Alcaligenes sp. XY-234
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.32
?
x * 59000, SDS-PAGE
Alcaligenes sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.32
40
-
-
Alcaligenes sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.32
40
-
pH 7.5, 10 min, stable below
Alcaligenes sp.
3.2.1.32
50
-
pH 7.5, 10 min, 70% loss of activity
Alcaligenes sp.
3.2.1.32
60
-
pH 7.5, 10 min, 99% loss of activity
Alcaligenes sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.32
7.5
-
-
Alcaligenes sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.32
4
-
4°C, 24 h, 35% loss of activity
Alcaligenes sp.
3.2.1.32
6
10
4°C, 24 h, enzyme retains 90-100% of activity
Alcaligenes sp.
3.2.1.32
12
-
4°C, 24 h, retains 70% of its activity
Alcaligenes sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.32
Ag+
1 mM, over 90% inhibition
Alcaligenes sp.
3.2.1.32
Al3+
1 mM AlCl3, strong
Alcaligenes sp.
3.2.1.32
Cu2+
1 mM CuCl2, strong
Alcaligenes sp.
3.2.1.32
Fe3+
1 mM FeCl3, strong
Alcaligenes sp.
3.2.1.32
Hg2+
1 mM, strong
Alcaligenes sp.
3.2.1.32
N-bromosuccinimide
-
Alcaligenes sp.
3.2.1.32
Pb(CH3COO)2
1 mM, strong
Alcaligenes sp.
3.2.1.32
Pb2+
1 mM, over 90% inhibition
Alcaligenes sp.
3.2.1.32
Zn2+
1 mM, over 90% inhibition
Alcaligenes sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.32
additional information
-
additional information
-
Alcaligenes sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.32
59000
-
x * 59000, SDS-PAGE
Alcaligenes sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.32
-
Alcaligenes sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.2.1.32
culture medium
-
Alcaligenes sp.
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.2.1.32
17.5
-
-
Alcaligenes sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.32
beta-1,3-xylan + H2O
-
136250
Alcaligenes sp.
xylose + xylooligosaccharides
-
136250
Alcaligenes sp.
?
3.2.1.32
beta-1,3-xylan + H2O
-
136250
Alcaligenes sp. XY-234
xylose + xylooligosaccharides
-
136250
Alcaligenes sp. XY-234
?
3.2.1.32
additional information
no hydrolysis of xylobiose and p-nitrophenyl-beta-D-xyloside
136250
Alcaligenes sp.
?
-
-
-
?
3.2.1.32
additional information
no hydrolysis of xylobiose and p-nitrophenyl-beta-D-xyloside
136250
Alcaligenes sp. XY-234
?
-
-
-
?
3.2.1.32
xylopentaose + H2O
-
136250
Alcaligenes sp.
xylose + xylotetraose + xylobiose + xylotriose
-
136250
Alcaligenes sp.
?
3.2.1.32
xylopentaose + H2O
-
136250
Alcaligenes sp. XY-234
xylose + xylotetraose + xylobiose + xylotriose
-
136250
Alcaligenes sp. XY-234
?
3.2.1.32
xylotetraose + H2O
-
136250
Alcaligenes sp.
xylose + xylotriose + xylobiose
-
136250
Alcaligenes sp.
?
3.2.1.32
xylotetraose + H2O
-
136250
Alcaligenes sp. XY-234
xylose + xylotriose + xylobiose
-
136250
Alcaligenes sp. XY-234
?
3.2.1.32
xylotriose + H2O
-
136250
Alcaligenes sp.
xylose + xylobiose
-
136250
Alcaligenes sp.
?
3.2.1.32
xylotriose + H2O
-
136250
Alcaligenes sp. XY-234
xylose + xylobiose
-
136250
Alcaligenes sp. XY-234
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.32
?
x * 59000, SDS-PAGE
Alcaligenes sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.32
40
-
-
Alcaligenes sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.32
40
-
pH 7.5, 10 min, stable below
Alcaligenes sp.
3.2.1.32
50
-
pH 7.5, 10 min, 70% loss of activity
Alcaligenes sp.
3.2.1.32
60
-
pH 7.5, 10 min, 99% loss of activity
Alcaligenes sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.32
7.5
-
-
Alcaligenes sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.32
4
-
4°C, 24 h, 35% loss of activity
Alcaligenes sp.
3.2.1.32
6
10
4°C, 24 h, enzyme retains 90-100% of activity
Alcaligenes sp.
3.2.1.32
12
-
4°C, 24 h, retains 70% of its activity
Alcaligenes sp.