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Literature summary extracted from

  • Chun, S.B.; Bai, S.; Im, S.Y.; Choi, W.K.; Lee, J.J.
    Purification and characterization of two extracellular glucoamylase isozymes from Lipomyces kononenkoae (1995), J. Biochem. Mol. Biol., 28, 375-381.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information 6.57 mg/ml for glucoamylase I with starch as substrate, 4.52 mg/ml for glucoamylase II with starch as substrate. 0.15 mg/ml for glucoamylase II with alpha-cyclodextrin as substrate, 2.0 mg/ml for glucoamylase II with beta-cyclodextrin as substrate Lipomyces kononenkoae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.3 extracellular
-
 Lipomyces kononenkoae
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 128000
-
 1 * 128000, glucoamylase II, SDS-PAGE Lipomyces kononenkoae
3.2.1.3 140000
-
 glucoamylase II, gel filtration Lipomyces kononenkoae
3.2.1.3 150000
-
 2 * 150000, glucoamylase I, SDS-PAGE Lipomyces kononenkoae
3.2.1.3 230000
-
 glucoamylase I, gel filtration Lipomyces kononenkoae

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Lipomyces kononenkoae
-
 CBS 5608 mutant
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein glucoamylase I contains 7.5% carbohydrate, glucoamylase II contains 6.5% carbohydrate Lipomyces kononenkoae

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 glucoamylase I and II Lipomyces kononenkoae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 alpha-cyclodextrin + H2O
-
 Lipomyces kononenkoae glucose + ?
-
 ?
3.2.1.3 beta-cyclodextrin + H2O
-
 Lipomyces kononenkoae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 dimer 2 * 150000, glucoamylase I, SDS-PAGE Lipomyces kononenkoae
3.2.1.3 monomer 1 * 128000, glucoamylase II, SDS-PAGE Lipomyces kononenkoae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 60
-
-
 Lipomyces kononenkoae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 50 60 stable Lipomyces kononenkoae
3.2.1.3 70
-
 rapid inactivation above Lipomyces kononenkoae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
-
 Lipomyces kononenkoae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 4 6.5 stable Lipomyces kononenkoae
3.2.1.3 5 8 stable Lipomyces kononenkoae