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Literature summary extracted from

  • Nagasaka, Y.; Kurosawa, K.; Yokota, A.; Tomita, F.
    Purification and properties of the raw-starch-digesting glucoamylases from Corticium rolfsii (1998), Appl. Microbiol. Biotechnol., 50, 323-330.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 0.0002
-
 starch soluble, glucoamylase G1 or G3 Athelia rolfsii
3.2.1.3 0.0002
-
 amylopectin glucoamylase G2 or G3 Athelia rolfsii
3.2.1.3 0.0003
-
 starch soluble, glucoamylase G2 Athelia rolfsii
3.2.1.3 0.0003
-
 amylopectin glucoamylase G1 Athelia rolfsii
3.2.1.3 0.0005
-
 glycogen glucoamylase G2 Athelia rolfsii
3.2.1.3 0.0006
-
 glycogen glucoamylase G3 Athelia rolfsii
3.2.1.3 0.0007
-
 glycogen glucoamylase G1 Athelia rolfsii
3.2.1.3 0.0021
-
 amylose DP = 18, glucoamylase G1, G2 or G3 Athelia rolfsii
3.2.1.3 0.025
-
 maltoheptaose glucoamylase G2 Athelia rolfsii
3.2.1.3 0.026
-
 maltoheptaose glucoamylase G1 Athelia rolfsii
3.2.1.3 0.027
-
 maltoheptaose glucoamylase G3 Athelia rolfsii
3.2.1.3 0.045
-
 maltohexaose glucoamylase G2 Athelia rolfsii
3.2.1.3 0.045
-
 starch soluble, glucoamylase G4 Athelia rolfsii
3.2.1.3 0.048
-
 maltohexaose glucoamylase G1 Athelia rolfsii
3.2.1.3 0.048
-
 starch soluble, glucoamylase G5 Athelia rolfsii
3.2.1.3 0.05
-
 maltohexaose glucoamylase G3 Athelia rolfsii
3.2.1.3 0.058
-
 maltoheptaose glucoamylase G5 Athelia rolfsii
3.2.1.3 0.059
-
 maltohexaose glucoamylase G4 Athelia rolfsii
3.2.1.3 0.059
-
 maltoheptaose glucoamylase G4 Athelia rolfsii
3.2.1.3 0.06
-
 maltohexaose glucoamylase G5 Athelia rolfsii
3.2.1.3 0.063
-
 amylose DP 18 glucoamylase G4 Athelia rolfsii
3.2.1.3 0.065
-
 amylose DP 18 glucoamylase G5 Athelia rolfsii
3.2.1.3 0.066
-
 maltopentaose glucoamylase G3 Athelia rolfsii
3.2.1.3 0.067
-
 maltopentaose glucoamylase G4 or G5 Athelia rolfsii
3.2.1.3 0.07
-
 maltopentaose glucoamylase G1 Athelia rolfsii
3.2.1.3 0.071
-
 maltopentaose glucoamylase G2 Athelia rolfsii
3.2.1.3 0.082
-
 maltotetraose glucoamylase G4 Athelia rolfsii
3.2.1.3 0.083
-
 maltotetraose glucoamylase G5 Athelia rolfsii
3.2.1.3 0.088
-
 maltotetraose glucoamylase G3 Athelia rolfsii
3.2.1.3 0.095
-
 maltotetraose glucoamylase G1 or G2 Athelia rolfsii
3.2.1.3 0.16
-
 amylopectin glucoamylase G5 Athelia rolfsii
3.2.1.3 0.17
-
 amylopectin glucoamylase G4 Athelia rolfsii
3.2.1.3 0.21
-
 maltotriose glucoamylase G1, G2 or G3 Athelia rolfsii
3.2.1.3 0.23
-
 maltotriose glucoamylase G4 Athelia rolfsii
3.2.1.3 0.24
-
 maltotriose glucoamylase G5 Athelia rolfsii
3.2.1.3 0.62
-
 glycogen glucoamylase G5 Athelia rolfsii
3.2.1.3 0.63
-
 glycogen glucoamylase G4 Athelia rolfsii
3.2.1.3 0.67
-
 maltose glucoamylase G4 and G5 Athelia rolfsii
3.2.1.3 0.68
-
 maltose glucoamylase G1 and G2 Athelia rolfsii
3.2.1.3 0.69
-
 maltose glucoamylase G3 Athelia rolfsii
3.2.1.3 15.4
-
 panose glucoamylase G1 Athelia rolfsii
3.2.1.3 15.8
-
 panose glucoamylase G2 Athelia rolfsii
3.2.1.3 16.7
-
 panose glucoamylase G4 Athelia rolfsii
3.2.1.3 16.9
-
 panose glucoamylase G5 Athelia rolfsii
3.2.1.3 17.2
-
 panose glucoamylase G3 Athelia rolfsii
3.2.1.3 20
-
 isomaltose glucoamylase G1 Athelia rolfsii
3.2.1.3 20.1
-
 isomaltose glucoamylase G2 Athelia rolfsii
3.2.1.3 20.4
-
 isomaltose glucoamylase G3 Athelia rolfsii
3.2.1.3 21.7
-
 isomaltose glucoamylase G4 Athelia rolfsii
3.2.1.3 23
-
 isomaltose glucoamylase G5 Athelia rolfsii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.3 57000
-
 x * 57000, glucoamylase G5, SDS-PAGE Athelia rolfsii
3.2.1.3 58000
-
 x * 58000, glucoamylase G4, SDS-PAGE Athelia rolfsii
3.2.1.3 69000
-
 1 * 69000, glucoamylase G5, SDS-PAGE Athelia rolfsii
3.2.1.3 70000
-
 1 * 70000, glucoamylase G4, SDS-PAGE Athelia rolfsii
3.2.1.3 72000
-
 x * 72000, glucoamylase 2, SDS-PAGE Athelia rolfsii
3.2.1.3 73000
-
 x * 73000, glucoamylase 1, SDS-PAGE Athelia rolfsii
3.2.1.3 74000
-
 glucoamylase G3, SDS-PAGE Athelia rolfsii
3.2.1.3 78000
-
 1 * 78000, glucoamylase 1 and 2, SDS-PAGE Athelia rolfsii
3.2.1.3 79000
-
 1 * 79000, glucoamylase G3, SDS-PAGE Athelia rolfsii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Athelia rolfsii
-
 AHU 9627
-
3.2.1.3 Athelia rolfsii AHU 9627
-
 AHU 9627
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.3 glycoprotein glucoamylase G1 contains 8.1% carbohydrate, glucoamylase G2 contains 7.6% carbohydrate, glucoamylase G3 contains 8.0% carbohydrate, glucoamylase G4 and G5 contain 7.5% carbohydrate Athelia rolfsii

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 5 forms of glucoamylase: G1-G5 Athelia rolfsii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 29
-
 enzyme form G4 Athelia rolfsii
3.2.1.3 38
-
 enzyme form G3 Athelia rolfsii
3.2.1.3 39
-
 enzyme form G2 Athelia rolfsii
3.2.1.3 41
-
 enzyme form G1 Athelia rolfsii
3.2.1.3 44
-
 enzyme form G4 Athelia rolfsii
3.2.1.3 47
-
 enzyme form G5 Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 amylopectin + H2O
-
 Athelia rolfsii D-glucose + ?
-
 ?
3.2.1.3 amylopectin + H2O
-
 Athelia rolfsii AHU 9627 D-glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Athelia rolfsii glucose + ?
-
 ?
3.2.1.3 amylose + H2O
-
 Athelia rolfsii AHU 9627 glucose + ?
-
 ?
3.2.1.3 amylose DP 18 + H2O
-
 Athelia rolfsii beta-D-glucose + amylose DP 17
-
 ?
3.2.1.3 glycogen + H2O
-
 Athelia rolfsii glucose + ?
-
 ?
3.2.1.3 glycogen + H2O
-
 Athelia rolfsii AHU 9627 glucose + ?
-
 ?
3.2.1.3 isomaltose + H2O
-
 Athelia rolfsii glucose
-
 ?
3.2.1.3 maltoheptaose + H2O
-
 Athelia rolfsii ?
-
 ?
3.2.1.3 maltohexaose + H2O
-
 Athelia rolfsii ?
-
 ?
3.2.1.3 maltopentaose + H2O
-
 Athelia rolfsii ?
-
 ?
3.2.1.3 maltose + H2O
-
 Athelia rolfsii 2 glucose
-
 ?
3.2.1.3 maltotetraose + H2O
-
 Athelia rolfsii ?
-
 ?
3.2.1.3 maltotriose + H2O
-
 Athelia rolfsii maltose + glucose
-
 ?
3.2.1.3 panose + H2O
-
 Athelia rolfsii ?
-
 ?
3.2.1.3 starch + H2O soluble Athelia rolfsii glucose + ?
-
 ?
3.2.1.3 starch + H2O raw starch and gelatinized starch Athelia rolfsii glucose + ?
-
 ?
3.2.1.3 starch + H2O soluble Athelia rolfsii AHU 9627 glucose + ?
-
 ?
3.2.1.3 starch + H2O raw starch and gelatinized starch Athelia rolfsii AHU 9627 glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 72000, glucoamylase 2, SDS-PAGE Athelia rolfsii
3.2.1.3 ? x * 74000, glucoamylase G3, SDS-PAGE Athelia rolfsii
3.2.1.3 ? x * 73000, glucoamylase 1, SDS-PAGE Athelia rolfsii
3.2.1.3 ? x * 58000, glucoamylase G4, SDS-PAGE Athelia rolfsii
3.2.1.3 ? x * 57000, glucoamylase G5, SDS-PAGE Athelia rolfsii
3.2.1.3 monomer 1 * 79000, glucoamylase G3, SDS-PAGE Athelia rolfsii
3.2.1.3 monomer 1 * 78000, glucoamylase 1 and 2, SDS-PAGE Athelia rolfsii
3.2.1.3 monomer 1 * 69000, glucoamylase G5, SDS-PAGE Athelia rolfsii
3.2.1.3 monomer 1 * 70000, glucoamylase G4, SDS-PAGE Athelia rolfsii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 65
-
 hydrolysis of raw starch, enzyme form G1, G2, and G3 Athelia rolfsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 55
-
 10 min, pH 4.0, enzyme form G1, G2, G3, G4 and G5, stable up to Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.3 3 8 30°C, 24 h, stable Athelia rolfsii