EC Number | General Stability | Organism |
---|---|---|
3.2.1.28 | Ca2+ and Mn2+ protect from thermoinactivation | Mycothermus thermophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.28 | ADP | - |
Mycothermus thermophilus | |
3.2.1.28 | Al3+ | 1 mM AlCl3, complete inhibition | Mycothermus thermophilus | |
3.2.1.28 | ATP | - |
Mycothermus thermophilus | |
3.2.1.28 | Cu2+ | 1 mM CuSO4, complete inhibition of extracellular enzyme, 47% inhibition of intracellular enzyme | Mycothermus thermophilus | |
3.2.1.28 | EDTA | 1 mM, 30% inhibition of extracellular enzyme, 54% inhibition of intracellular enzyme | Mycothermus thermophilus | |
3.2.1.28 | Hg2+ | 1 mM HgCl2, complete inhibition | Mycothermus thermophilus | |
3.2.1.28 | NH4Cl | 1 mM NH4Cl, 22% inhibition of extracellular enzyme, 27% inhibition of intracellular enzyme | Mycothermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.28 | 2.24 | - |
trehalose | intracellular enzyme | Mycothermus thermophilus | |
3.2.1.28 | 3.58 | - |
trehalose | extracellular enzyme | Mycothermus thermophilus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.28 | extracellular | intracellular and extracellular enzyme | Mycothermus thermophilus | - |
- |
3.2.1.28 | intracellular | intracellular and extracellular enzyme | Mycothermus thermophilus | 5622 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.28 | Ca2+ | activates | Mycothermus thermophilus | |
3.2.1.28 | Co2+ | slight stimulation | Mycothermus thermophilus | |
3.2.1.28 | Mn2+ | activates | Mycothermus thermophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 82000 | - |
5 * 82000, extracellular enzyme, SDS-PAGE | Mycothermus thermophilus |
3.2.1.28 | 85000 | - |
5 * 85000, intracellular enzyme, SDS-PAGE | Mycothermus thermophilus |
3.2.1.28 | 370000 | - |
extracellular enzyme, gel filtration | Mycothermus thermophilus |
3.2.1.28 | 398000 | - |
intracellular enzyme, gel filtration | Mycothermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.28 | Mycothermus thermophilus | - |
intracellular and extracellular threhalase | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.28 | glycoprotein | extracellular enzyme contains 81% carbohydrate, intracellular enzyme contains 51% carbohydrate | Mycothermus thermophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 1775 | - |
extracellular enzyme | Mycothermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.28 | trehalose + H2O | absolute specificity | Mycothermus thermophilus | D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.28 | pentamer | 5 * 82000, extracellular enzyme, SDS-PAGE | Mycothermus thermophilus |
3.2.1.28 | pentamer | 5 * 85000, intracellular enzyme, SDS-PAGE | Mycothermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 60 | - |
extracellular enzyme | Mycothermus thermophilus |
3.2.1.28 | 65 | - |
intracellular enzyme | Mycothermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 60 | - |
extracellular enzyme has a half-life of 3 min. Intracellular enzyme loses 20% of activity after 40 min when incubated without Ca2+, and is fully stable in presence of Ca2+ | Mycothermus thermophilus |
3.2.1.28 | 65 | - |
intracellular enzyme has a half-life of 4.5 min when incubated without Ca2+ | Mycothermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.28 | 6 | - |
extracellular and intracellular enzyme | Mycothermus thermophilus |