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Literature summary extracted from

  • Tulsiani, D.R.P.; Hubbard, S.C.; Robbins, P.W.; Touster, O.
    alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates (1982), J. Biol. Chem., 257, 3660-3668.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.113 mannosidase IA and B differ in sensitivity to storage in liver cytoplasmic extracts, no interconversion between mannosidase IA and IB during cellulose phosphate chromatography Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 Co2+ inhibition of alpha-mannosidases IA and IB Rattus sp.
3.2.1.24 Cu2+ inhibition of alpha-mannosidases IA, IB and II Rattus sp.
3.2.1.24 EDTA inhibitory for alpha-mannosidases IA and IB Rattus sp.
3.2.1.24 methyl-alpha-D-mannopyranoside slight inhibitory for alpha-mannosidases IA and IB Rattus sp.
3.2.1.24 p-chloromercuribenzenesulfonic acid different inhibition rates for alpha-mannosidases IA, IB and II Rattus sp.
3.2.1.24 Tris inhibitory for alpha-mannosidases IA and IB Rattus sp.
3.2.1.24 Zn2+ slight inhibition for alpha-mannosidase IA Rattus sp.
3.2.1.113 additional information mannosidase IA and B are quite similar in response to inhibitors Rattus norvegicus
3.2.1.114 Cu2+ 1 mM, 91-93% inhibition; strong Rattus norvegicus
3.2.1.114 Fe2+ 1 mM, 70-76% inhibition; strong Rattus norvegicus
3.2.1.114 Fe3+ less potent inhibitor than Fe2+ and Cu2+, 1 mM, 24-30% inhibition Rattus norvegicus
3.2.1.114 additional information not inhibited by Co2+, Zn2+; not inhibited by EDTA; not inhibited by Tris-Cl, iodoacetamide, alpha-methylmannoside; not inhibited by Tris-maleate Rattus norvegicus
3.2.1.114 p-chloromercuriphenylsulfonate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 Golgi apparatus 3 isoforms, alpha-mannosidases IA, IB and II Rattus sp. 5794
-
3.2.1.24 membrane from Golgi-enriched membrane fraction Rattus sp. 16020
-
3.2.1.113 Golgi membrane
-
 Rattus norvegicus 139
-
3.2.1.113 membrane
-
 Rattus norvegicus 16020
-
3.2.1.114 Golgi membrane
-
 Rattus norvegicus 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.24 Co2+ slight activation for alpha-mannosidase II Rattus sp.
3.2.1.24 Zn2+ slight activation for alpha-mannosidase II Rattus sp.
3.2.1.114 Co2+ activation Rattus norvegicus
3.2.1.114 Zn2+ activation Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 Man9-5GlcNAc + 4 H2O Rattus sp. different product composition for alpha-mannosidases IA, IB and II Man5-3GlcNAc + 4 alpha-D-mannopyranose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Rattus norvegicus
-
 (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Rattus norvegicus Wistar
-
 (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.114 additional information Rattus norvegicus biosynthesis of complex-type N-linked oligosaccharides of glycoproteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Rattus sp.
-
-
-
3.2.1.113 Rattus norvegicus
-
 male
-
3.2.1.113 Rattus norvegicus
-
 Wistar
-
3.2.1.113 Rattus norvegicus Wistar
-
 Wistar
-
3.2.1.114 Rattus norvegicus
-
 male Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Rattus sp.
3.2.1.113 partial Rattus norvegicus
3.2.1.114 20fold Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 liver
-
 Rattus sp.
-
3.2.1.113 liver
-
 Rattus norvegicus
-
3.2.1.114 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.113 additional information
-
-
 Rattus norvegicus
3.2.1.114 1.425
-
 4-nitrophenyl alpha-D-mannoside as substrate Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.2.1.113 2-4°C, in 10 mM potassium phosphate buffer, pH 7.2, 1% Triton X-100, 20% loss within 4 weeks Rattus norvegicus
3.2.1.114 2-4°C, 10 mM potassium phosphate buffer, pH 7.2, 1% Triton X-100, 4 weeks, 20% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 Man9-5GlcNAc + 4 H2O different product composition for alpha-mannosidases IA, IB and II Rattus sp. Man5-3GlcNAc + 4 alpha-D-mannopyranose
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O isoforms mannosidase IB and II Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?
3.2.1.113 GlcNAc-(Man)5GlcNAc + H2O poor substrate Rattus norvegicus ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Rattus norvegicus (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O best substrate Rattus norvegicus (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Rattus norvegicus Wistar (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O best substrate Rattus norvegicus Wistar (Man)5GlcNAc + 4 D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O hydrolysis at 39% (mannosidase IB) or 67% (mannosidase IA) the rate of (Man)9GlcNAc hydrolysis Rattus norvegicus ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-2)Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNA + H2O hydrolysis at 39% (mannosidase IB) or 67% (mannosidase IA) the rate of (Man)9GlcNAc hydrolysis Rattus norvegicus Wistar ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O hydrolysis at 18-25% the rate of (Man)9GlcNAc hydrolysis Rattus norvegicus ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-Asn + H2O hydrolysis at 18-25% the rate of (Man)9GlcNAc hydrolysis Rattus norvegicus Wistar ? + D-mannose
-
 ?
3.2.1.113 Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAc + H2O without alpha1,2-linked mannose residues: poor substrate Rattus norvegicus (Man)4GlcNAc + (Man)3GlcNAc + D-mannose
-
 ?
3.2.1.113 additional information mannosidase IA and B are quite similar in substrate specificity Rattus norvegicus ?
-
 ?
3.2.1.113 additional information mannosidase IA and B are quite similar in substrate specificity Rattus norvegicus Wistar ?
-
 ?
3.2.1.113 p-nitrophenyl-alpha-D-mannoside + H2O mannosidase IB: poor substrate, mannosidase IA: no substrate Rattus norvegicus 4-nitrophenol + D-mannose
-
 ?
3.2.1.114 (Man)6GlcNAc + H2O rate at 2% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
3.2.1.114 (Man)7GlcNAc + H2O rate at 3% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
3.2.1.114 (Man)8GlcNAc + H2O rate at 5% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
3.2.1.114 (Man)9GlcNAc + H2O rate at 6.4% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus ?
-
 ?
3.2.1.114 4-nitrophenyl alpha-D-mannoside + H2O rate at 23.5% of GlcNAc(Man)5GlcNAc hydrolysis Rattus norvegicus 4-nitrophenol + alpha-D-mannose
-
 ?
3.2.1.114 D-Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Rattus norvegicus D-Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + alpha-D-mannose
-
 ?
3.2.1.114 GlcNAc(Man)5GlcNAc + 2 H2O preferred substrate Rattus norvegicus GlcNAc(Man)3GlcNAc + 2 alpha-D-mannose
-
 ?
3.2.1.114 additional information not: (Man)5GlcNAc Rattus norvegicus ?
-
 ?
3.2.1.114 additional information biosynthesis of complex-type N-linked oligosaccharides of glycoproteins Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.24 Golgi alpha-mannosidase II
-
 Rattus sp.
3.2.1.24 Golgi mannosidase IA
-
 Rattus sp.
3.2.1.24 Golgi mannosidase IB
-
 Rattus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.113 37
-
 assay at Rattus norvegicus
3.2.1.114 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.113 additional information
-
 mannosidase IA and B differ in thermostability Rattus norvegicus
3.2.1.113 37
-
 24 h, in 0.1 M sodium acetate buffer, pH 5.8, 30% loss of activity Rattus norvegicus
3.2.1.113 50
-
 mannosidase IB less stable than IA Rattus norvegicus
3.2.1.114 37
-
 24 h, in 100 mM sodium acetate buffer, pH 5.8, 30-50% loss of activity Rattus norvegicus
3.2.1.114 50
-
 3 h, about 30% loss of activity, 4-nitrophenyl alpha-D-mannoside or (Man)9GlcNAc as substrate Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 5.5
-
 alpha-mannosidase II with p-nitrophenyl-alpha-D-mannopyranoside as substrate Rattus sp.
3.2.1.24 5.8
-
 alpha-mannosidase II with Man8GlcNAc as substrate Rattus sp.
3.2.1.24 6
-
 alpha-mannosidases IA and IB with Man8GlcNAc as substrate Rattus sp.
3.2.1.113 6
-
 mannosidase IA and B Rattus norvegicus
3.2.1.114 5.5
-
 4-nitrophenyl alpha-D-mannoside hydrolysis Rattus norvegicus
3.2.1.114 5.8
-
 (Man)8GlcNAc as substrate Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.114 4
-
 1 h, at 37°C, inactivation, 1 mM ZnCl2 partially stabilizes Rattus norvegicus
3.2.1.114 6
-
 1 h, at 37°C, 30% loss of activity Rattus norvegicus
3.2.1.114 8
-
 1 h, at 37°C, stable Rattus norvegicus