Any feedback?
Literature summary extracted from
- Co2+-mediated time- and temperature-dependend activation of neutral alpha-D-mannosidase from monkey brain (1988), Biochem. J., 253, 677-685.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.2.1.24 | stable in the presence of Co2+ at 37°C | Macaca radiata |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | 2-amino-2-deoxy-D-glucose | competitive inhibition, Ki = 2.8 mM | Macaca radiata |  |
| 3.2.1.24 | D-mannosylamine | 15% competitive inhibition at 5 mM | Macaca radiata | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.24 | Co2+ | activation after preincubation at 1.4 mM, activation persists even after removal of Co2+ | Macaca radiata | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 45000 | - |
SDS-PAGE | Macaca radiata |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Macaca radiata | - |
monkey | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | near homogeneity, preincubation with Co2+, metal chelating affinity chromatography | Macaca radiata |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | brain | - |
Macaca radiata | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 0.057 | - |
- |
Macaca radiata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | 4-methylumbelliferyl-alpha-D-mannopyranoside + H2O | - |
Macaca radiata | 4-methylumbelliferone + alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Macaca radiata | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | neutral alpha-mannosidase | - |
Macaca radiata |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.24 | 2.8 | - |
2-amino-2-deoxy-D-glucose | - |
Macaca radiata | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
