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Literature summary extracted from

  • Tulsiani, D.R.P.; Skudlarek, M.D.; Nagdas, S.K.; Orgebin-Crist, M.C.
    Purification and characterization of rat epididymal-fluid alpha-D-mannosidase: similarities to sperm plasma-membrane alpha-D-mannosidase (1993), Biochem. J., 290, 427-436.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.24 alpha-D-mannopyranose 27% inhibition at 10 mM Rattus sp.
3.2.1.24 D-mannosylamine 56% inhibition at 1 mM Rattus sp.
3.2.1.24 Mannan 33% inhibition at 50 mM Rattus sp.
3.2.1.24 methyl-alpha-D-glucoside 23% inhibition at 10 mM Rattus sp.
3.2.1.24 methyl-alpha-D-mannopyranoside 41% inhibition at 10 mM Rattus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.24 Golgi lumen luminal fluid Rattus sp. 5796
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.24 115000
-
 4 * 115000, SDS-PAGE Rattus sp.
3.2.1.24 460000
-
 gel filtration Rattus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.24 GlcNAcMan5GlcNAc + H2O Rattus sp. low activity alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 glycoprotein + H2O Rattus sp.
-
 alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Man-glycopeptide + H2O Rattus sp. low activity alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Man5-9GlcNAc + H2O Rattus sp. low activity for Man5GlcNAc alpha-D-mannopyranose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.24 Rattus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.24 to homogeneity, chromatography steps Rattus sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.24 epididymis cauda epididymis Rattus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.24 20.52
-
-
 Rattus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.24 GlcNAcMan5GlcNAc + H2O low activity Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 glycoprotein + H2O
-
 Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Man-glycopeptide + H2O low activity Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 Man5-9GlcNAc + H2O low activity for Man5GlcNAc Rattus sp. alpha-D-mannopyranose + ?
-
 ?
3.2.1.24 p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
 Rattus sp. p-nitrophenol + alpha-D-mannopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.24 tetramer 4 * 115000, SDS-PAGE Rattus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.24
-
 4 60-70% loss of activity within 24 h Rattus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.24 6.2 6.5
-
 Rattus sp.