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Literature summary extracted from
- Purification, crystallization and preliminary X-ray crystallographic analysis of recombinant murine Golgi mannosidase IA, a class I alpha-mannosidase involved in Asn-linked oligosaccharide maturation (1999), Acta Crystallogr. Sect. D, 55, 571-573.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | expression in Pichia pastoris | Mus musculus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | crystal structure at 2.8 A resolution | Mus musculus |
| 3.2.1.113 | - |
Mus musculus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.24 | membrane | - |
Mus musculus | 16020 | - |
| 3.2.1.113 | membrane | type II membrane protein | Mus musculus | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.24 | 53000 | - |
SDS-PAGE | Mus musculus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | Man8GlcNAc2 + 3 H2O | Mus musculus | - |
Man5GlcNAc2 + 3 alpha-D-mannopyranose | - |
? |  |
| 3.2.1.24 | Man9GlcNAc2 + H2O | Mus musculus | - |
Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose | major product: Man5GlcNAc2, involved in Asn-linked oligosaccharide maturation | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.24 | Mus musculus | - |
- |
- |
| 3.2.1.113 | Mus musculus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.24 | recombinant protein, to homogeneity | Mus musculus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.24 | Man8GlcNAc2 + 3 H2O | - |
Mus musculus | Man5GlcNAc2 + 3 alpha-D-mannopyranose | - |
? | |
| 3.2.1.24 | Man9GlcNAc2 + H2O | - |
Mus musculus | Man5GlcNAc2 + Man6GlcNAc2 + Man7GlcNAc2 + Man8GlcNAc2 + alpha-D-mannopyranose | major product: Man5GlcNAc2, involved in Asn-linked oligosaccharide maturation | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | monomer | 1 * 53000, crystallographic analysis | Mus musculus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.24 | Golgi mannosidase IA | - |
Mus musculus |
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