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Literature summary extracted from

  • Miyairi, K.; Okuno, T.; Sawai, K.
    Purification and properties of endopolygalacturonase I from Stereum purpureum, a factor inducing silver-leaf symptoms on apple trees (1985), Agric. Biol. Chem., 49, 111-118.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Ba2+
-
 Chondrostereum purpureum
3.2.1.15 Cd2+
-
 Chondrostereum purpureum
3.2.1.15 Hg2+ 0.5 mM, complete inhibition Chondrostereum purpureum
3.2.1.15 Sn2+
-
 Chondrostereum purpureum
3.2.1.15 Sr2+
-
 Chondrostereum purpureum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.15 Na+ 50 mM, stimulates Chondrostereum purpureum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 40000
-
 gel filtration Chondrostereum purpureum
3.2.1.15 41000
-
 1 * 41000, SDS-PAGE Chondrostereum purpureum
3.2.1.15 44000
-
 equilibrium sedimentation Chondrostereum purpureum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.15 additional information Chondrostereum purpureum the enzyme is a factor inducing silver-leaf symptoms on apple trees ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Chondrostereum purpureum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15
-
 Chondrostereum purpureum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 560
-
-
 Chondrostereum purpureum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Chondrostereum purpureum digalacturonic acid + galacturonate
-
 ?
3.2.1.15 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Chondrostereum purpureum alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + D-galacturonate
-
 ?
3.2.1.15 additional information the enzyme is a factor inducing silver-leaf symptoms on apple trees Chondrostereum purpureum ?
-
 ?
3.2.1.15 pectic acid + H2O
-
 Chondrostereum purpureum galacturonic acid oligomers
-
 ?
3.2.1.15 polygalacturonate + H2O
-
 Chondrostereum purpureum oligogalacturonates
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 monomer 1 * 41000, SDS-PAGE Chondrostereum purpureum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 70
-
-
 Chondrostereum purpureum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 40 90 40°C: about 40% of maximal activity, 90°C: about 35% of maximal activity Chondrostereum purpureum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 70
-
 30 min, stable up to Chondrostereum purpureum
3.2.1.15 75
-
 rapid inactivation Chondrostereum purpureum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 3.5
-
 hydrolysis of trigalacturonic acid Chondrostereum purpureum
3.2.1.15 4
-
 hydrolysis of tetragalacturonic acid Chondrostereum purpureum
3.2.1.15 4.5
-
 hydrolysis of pectic acid Chondrostereum purpureum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 4 9 30 min, stable up to 70°C Chondrostereum purpureum