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Literature summary extracted from

  • Nakajima, T.; Ballou, C.E.
    Structure of the linkage region between the polysaccharide and protein parts of Saccharomyces cerevisiae mannan (1974), J. Biol. Chem., 249, 7685-7694.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.101 analysis used to determine the structure of mannan-protein linkage region Niallia circulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.101 extracellular
-
 Niallia circulans
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.101 Niallia circulans
-
-
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.101 -10°C, crude enzyme solution, 0.05 M citrate-phosphate buffer, pH 5.5, 0.001 mM CaCl2 Niallia circulans
3.2.1.101 4°C, crude enzyme solution, 0.1 M citrate-phosphate buffer, pH 5.5, more than 1 month, without appreciable loss of activity Niallia circulans
3.2.1.101 frozen and lyophilized, crude enzyme solution, more than 1 month, without appreciable loss of activity Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.101 alpha-1,6-D-mannan + H2O Saccharomyces cerevisiae X2180-1A-5 mannan Niallia circulans D-mannose + alpha-1,6-D-mannobiose
-
 ?
3.2.1.101 additional information
-
 Niallia circulans ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.101 50
-
-
 Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.101 5.5 7
-
 Niallia circulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.101 5 7.5
-
 Niallia circulans