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Literature summary extracted from

  • Freeman, C.; Hopwood, J.J.
    Human glucosamine-6-sulphatase deficiency. Diagnostic enzymology towards heparin-derived trisaccharide substrates (1992), Biochem. J., 282, 605-614.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.6.14 bovine serum albumin stimulates only in urine at pH 3.9 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.14 0.0005 0.004 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in urine depending on pH and presence of bovine serum albumin Homo sapiens
3.1.6.14 0.009 0.017 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in fibroblasts Homo sapiens
3.1.6.14 0.028
-
 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in leucocytes Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.14 78000
-
 6 individual activites determined by chromatofocusing Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.14 N-acetyl-D-glucosamine 6-sulfate units + H2O Homo sapiens in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate N-acetyl-D-glucosamine units + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.14 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.14 6 individual activites determined Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.14 leukocyte
-
 Homo sapiens
-
3.1.6.14 liver
-
 Homo sapiens
-
3.1.6.14 skin fibroblast in culture Homo sapiens
-
3.1.6.14 urine
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.14 N-acetyl-D-glucosamine 6-sulfate units + H2O
-
 Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
3.1.6.14 N-acetyl-D-glucosamine 6-sulfate units + H2O in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
3.1.6.14 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate + H2O N-sulfated and N-acetylated derivates of Homo sapiens O-(alpha-glucosamine)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate + sulfate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.14 3.9
-
 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in fibroblasts Homo sapiens
3.1.6.14 4.1
-
 O-(alpha-glucosamine 6-sulfate)-(1-4)-L-O-(alpha-iduronic acid 2-sulfate)-(1-4)-D-O-2,5-anhydro mannitol 6-sulfate in leucocytes Homo sapiens