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Literature summary extracted from

  • Freeman, C.; Hopwood, J.J.
    Sanfilippo D syndrome: estimation of N-acetylglucosamine-6-sulfatase activity with a radiolabeled monosulfated disaccharide substrate (1989), Anal. Biochem., 176, 244-248.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.6.14 dithiothreitol stimulates only the purified enzyme Homo sapiens

Application

EC Number Application Comment Organism
3.1.6.14 medicine Sanfilippo D Syndrome: N-acetylglucosamine 6-sulfate sulfatase deficiency, mucopolysaccharidosis type IIID Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.6.14 bovine serum albumin inhibits only the purified enzyme Homo sapiens
3.1.6.14 NaCl
-
 Homo sapiens
3.1.6.14 PO43-
-
 Homo sapiens
3.1.6.14 SO42-
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.6.14 0.0072
-
 O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid in fibroblasts Homo sapiens
3.1.6.14 0.0153
-
 O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid in leucocytes Homo sapiens
3.1.6.14 0.027
-
 O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid in urine Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.14 N-acetyl-D-glucosamine 6-sulfate units + H2O Homo sapiens in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate N-acetyl-D-glucosamine units + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.14 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.14 amnion amniotic cell culture Homo sapiens
-
3.1.6.14 chorionic villus in culture Homo sapiens
-
3.1.6.14 leukocyte
-
 Homo sapiens
-
3.1.6.14 skin fibroblast in culture Homo sapiens
-
3.1.6.14 urine
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.14 N-acetyl-D-glucosamine 6-sulfate units + H2O
-
 Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
3.1.6.14 N-acetyl-D-glucosamine 6-sulfate units + H2O in oligo-and monosaccharides derived from heparan sulfate and keratan sulfate Homo sapiens N-acetyl-D-glucosamine units + sulfate
-
 ?
3.1.6.14 O-(alpha-N-acetyl glucosamine 6-sulfate)-(1-3)-L-idonic acid + H2O
-
 Homo sapiens O-(alpha-N-acetyl glucosamine)-(1-3)-L-idonic acid + sulfate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.6.14 4.2
-
 in fibroblasts Homo sapiens
3.1.6.14 4.5
-
 in leukocytes Homo sapiens
3.1.6.14 5.1
-
 in urine Homo sapiens