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Literature summary extracted from

  • Gheesling Mullis, K.; Huynh, M.; Kornfeld, R.H.
    Purification and kinetic parameters of bovine liver N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (1994), J. Biol. Chem., 269, 1718-1726.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.45 CdSO4 inhibits activity by 70% Bos taurus
3.1.4.45 CdSO4
-
 Homo sapiens
3.1.4.45 dithiothreitol
-
 Bos taurus
3.1.4.45 dithiothreitol 10 mM 15 min at 37°C inhibits activity by 90% Homo sapiens
3.1.4.45 MgCl2 inhibits activity by 30% Bos taurus
3.1.4.45 MnCl2 inhibits activity by 30% Bos taurus
3.1.4.45 N-acetylglucosamine
-
 Bos taurus
3.1.4.45 N-acetylglucosamine
-
 Homo sapiens
3.1.4.45 N-acetylglucosamine phosphate
-
 Bos taurus
3.1.4.45 N-acetylglucosamine phosphate
-
 Homo sapiens
3.1.4.45 PO43-
-
 Bos taurus
3.1.4.45 PO43-
-
 Homo sapiens
3.1.4.45 SDS 0.02% SDS inhibits activity by 14% Bos taurus
3.1.4.45 UDP-GlcNAc
-
 Bos taurus
3.1.4.45 UDP-GlcNAc
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.45 0.734
-
 UDP-GlcNAc
-
 Bos taurus
3.1.4.45 0.74
-
 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.45 69000
-
-
 Homo sapiens
3.1.4.45 69000
-
 fetal calf serum, reducing SDS-PAGE Bos taurus
3.1.4.45 121000
-
 liver, nonreducing SDS-PAGE Bos taurus
3.1.4.45 129000
-
 liver, nonreducing SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.45 Bos taurus
-
-
-
3.1.4.45 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.45
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
3.1.4.45 -20°C, superose 6 eluate, 3 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.45 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate
-
 ?
3.1.4.45 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O
-
 Bos taurus N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate
-
 ?
3.1.4.45 UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Homo sapiens N-acetyl-D-glucosamine + UDP
-
 ?
3.1.4.45 UDP-N-acetyl-alpha-D-glucosamine + H2O
-
 Bos taurus N-acetyl-D-glucosamine + UDP
-
 ?
3.1.4.45 UDP-N-acetyl-D-glucosamine + H2O
-
 Bos taurus UDP + N-acetyl-D-glucosamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.45 37
-
 assay at Homo sapiens
3.1.4.45 37
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.45 50
-
 after 3 min 80% of activity Bos taurus
3.1.4.45 75
-
 after 3 min only 7.6% activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.45 6.7
-
-
 Homo sapiens
3.1.4.45 6.7
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.45 6.5 9 liver Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.45 4 10 at pH 4, 54% of activity, at pH 10, 83% of activity Bos taurus