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Literature summary extracted from

  • Kornfeld, R.; Bao, M.; Brewer, K.; Noll, C.; Canfield, W.
    Purification and multimeric structure of bovine N-acetylglucosamine-phosphodiester alpha-N-acetylglucosaminidase (1998), J. Biol. Chem., 273, 23203-23210.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.45 0.49
-
 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.45 68000
-
 liver, reducing SDS-PAGE Bos taurus
3.1.4.45 136000
-
 liver, nonreducing SDS-PAGE Bos taurus
3.1.4.45 270000
-
 fetal calf serum, gel filtration Bos taurus
3.1.4.45 272000
-
 liver, gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.45 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.45 glycoprotein
-
 Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.45
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.45 liver
-
 Bos taurus
-
3.1.4.45 serum
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
3.1.4.45 -80°C, 20% glycerol, pH 7.4, 6 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.45 methyl 6-O-(N-acetyl-alpha-D-glucosaminyl)phospho-alpha-D-mannose + H2O
-
 Bos taurus N-acetyl-D-glucosamine + methyl alpha-D-mannose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.45 tetramer alpha4, 4 * 68000, reducing SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.45 37
-
 assay at Bos taurus