BRENDA - Enzyme Database

Purification of phospholipase D from peanuts

Tzur, R.; Shapiro, B.; Biochim. Biophys. Acta 280, 290-296 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
ethyl ether
-
Arachis hypogaea
3.1.4.4
phosphatidic acid
only with addition of lecithin and SDS
Arachis hypogaea
3.1.4.4
SDS
-
Arachis hypogaea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
albumin
-
Arachis hypogaea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
2.2
-
phosphatidylcholine
-
Arachis hypogaea
3.1.4.4
2.5
-
phosphatidylcholine
transphosphaditylation in presence of methanol
Arachis hypogaea
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
20-100 mM required for maximal activity in plants
Arachis hypogaea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Arachis hypogaea
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Arachis hypogaea
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Arachis hypogaea
-
peanut
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.4.4
partial
Arachis hypogaea
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
234
-
-
Arachis hypogaea
Storage Stability
EC Number
Storage Stability
Organism
3.1.4.4
-20°C, lyophilyzed, 2 month, no loss of activity
Arachis hypogaea
3.1.4.4
4°C, neutral pH, 50 days, no loss of activity
Arachis hypogaea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + ethanolamine
-
135438
Arachis hypogaea
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135438
Arachis hypogaea
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + methanol
-
135438
Arachis hypogaea
phosphatidylmethanol + choline
-
-
-
?
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135438
Arachis hypogaea
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135438
Arachis hypogaea
phosphatidic acid + alcohol
-
-
-
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
ethyl ether
-
Arachis hypogaea
3.1.4.4
phosphatidic acid
only with addition of lecithin and SDS
Arachis hypogaea
3.1.4.4
SDS
-
Arachis hypogaea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
albumin
-
Arachis hypogaea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
2.2
-
phosphatidylcholine
-
Arachis hypogaea
3.1.4.4
2.5
-
phosphatidylcholine
transphosphaditylation in presence of methanol
Arachis hypogaea
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
20-100 mM required for maximal activity in plants
Arachis hypogaea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.4.4
phospholipid + alcohol
Arachis hypogaea
transphosphaditylation
phospholipid + alcohol
-
-
?
3.1.4.4
phospholipid + H2O
Arachis hypogaea
phosphoric ester hydrolysis
phosphatidic acid + alcohol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.4.4
partial
Arachis hypogaea
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.4
234
-
-
Arachis hypogaea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.1.4.4
-20°C, lyophilyzed, 2 month, no loss of activity
Arachis hypogaea
3.1.4.4
4°C, neutral pH, 50 days, no loss of activity
Arachis hypogaea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + ethanolamine
-
135438
Arachis hypogaea
phosphatidylethanolamine + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + H2O
-
135438
Arachis hypogaea
1,2-diacylglycerophosphate + choline
-
-
-
?
3.1.4.4
phosphatidylcholine + methanol
-
135438
Arachis hypogaea
phosphatidylmethanol + choline
-
-
-
?
3.1.4.4
phospholipid + alcohol
transphosphaditylation
135438
Arachis hypogaea
phospholipid + alcohol
-
-
-
?
3.1.4.4
phospholipid + H2O
phosphoric ester hydrolysis
135438
Arachis hypogaea
phosphatidic acid + alcohol
-
-
-
?