EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.3 | sodium cholate | - |
Clostridium perfringens | |
3.1.4.3 | sodium dehydrocholate | - |
Clostridium perfringens |
EC Number | General Stability | Organism |
---|---|---|
3.1.4.3 | 1,10-phenanthroline, denaturation | Clostridium perfringens |
3.1.4.3 | sodium dodecyl sulfate | Clostridium perfringens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.3 | cysteine | - |
Clostridium perfringens | |
3.1.4.3 | EDTA | - |
Clostridium perfringens | |
3.1.4.3 | ferricyanide | - |
Clostridium perfringens | |
3.1.4.3 | fluoride | - |
Clostridium perfringens | |
3.1.4.3 | glutathione | - |
Clostridium perfringens | |
3.1.4.3 | o-phenanthroline | - |
Clostridium perfringens | |
3.1.4.3 | phosphinate analogue of lecithin | competitive inhibitor | Clostridium perfringens | |
3.1.4.3 | phosphonate analogue of lecithin | competitive inhibitor | Clostridium perfringens | |
3.1.4.3 | putrescine | - |
Clostridium perfringens | |
3.1.4.3 | SDS | - |
Clostridium perfringens | |
3.1.4.3 | spermidine | - |
Clostridium perfringens | |
3.1.4.3 | spermine | - |
Clostridium perfringens | |
3.1.4.3 | thioglycolic acid | - |
Clostridium perfringens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.3 | 0.016 | 1.2 | lecithin | - |
Clostridium perfringens | |
3.1.4.3 | 200 | - |
p-Nitrophenylphosphorylcholine | - |
Clostridium perfringens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.3 | extracellular | - |
Clostridium perfringens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.3 | Ca2+ | - |
Clostridium perfringens | |
3.1.4.3 | Co2+ | - |
Clostridium perfringens | |
3.1.4.3 | Mg2+ | - |
Clostridium perfringens | |
3.1.4.3 | Mn2+ | - |
Clostridium perfringens | |
3.1.4.3 | Zn2+ | - |
Clostridium perfringens | |
3.1.4.3 | Zn2+ | metalloenzyme | Clostridium perfringens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.3 | 31000 | - |
gel filtration | Clostridium perfringens |
3.1.4.3 | 43000 | - |
SDS-PAGE | Clostridium perfringens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.3 | 1,2-diacyl-sn-glycero-3-phosphatide + H2O | Clostridium perfringens | - |
1,2-diacylglycerol + phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.3 | Clostridium perfringens | - |
- |
- |
3.1.4.3 | Clostridium perfringens PB6K | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.3 | affinity chromatography, gel filtration, 2500fold purification from culture filtrate | Clostridium perfringens |
3.1.4.3 | column chromatography, gel filtration | Clostridium perfringens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.3 | 1620 | - |
substrate: lecithin | Clostridium perfringens |
EC Number | Storage Stability | Organism |
---|---|---|
3.1.4.3 | -20°C, 70 mg crude enzyme/ml, stable for at least a year | Clostridium perfringens |
3.1.4.3 | -20°C, more than 1-2 mg purified enzyme/ml, stable for at least 3 months | Clostridium perfringens |
3.1.4.3 | -20°C, stable for more than 6 months, crude enzyme | Clostridium perfringens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.3 | 1,2-diacyl-sn-glycero-3-phosphatide + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphate | - |
? | |
3.1.4.3 | ceramide aminoethylphosphonate | - |
Clostridium perfringens | N-acylsphingosine + aminoethylphosphonate | - |
? | |
3.1.4.3 | ceramide phosphorylethanolamine | - |
Clostridium perfringens | N-acylsphingosine + phosphorylethanolamine | - |
? | |
3.1.4.3 | choline plasmalogen | - |
Clostridium perfringens | ? | - |
? | |
3.1.4.3 | lysophosphatidylcholine + H2O | - |
Clostridium perfringens | monoacylglycerol + phosphorylcholine | - |
? | |
3.1.4.3 | lysophosphatidylcholine + H2O | - |
Clostridium perfringens PB6K | monoacylglycerol + phosphorylcholine | - |
? | |
3.1.4.3 | p-nitrophenylphosphoryl-choline + H2O | - |
Clostridium perfringens | p-nitrophenol + phosphorylcholine | - |
? | |
3.1.4.3 | phosphatidylcholine + H2O | - |
Clostridium perfringens | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
3.1.4.3 | phosphatidylcholine + H2O | - |
Clostridium perfringens PB6K | 1,2-diacylglycerol + phosphorylcholine | - |
? | |
3.1.4.3 | phosphatidylethanolamine + H2O | - |
Clostridium perfringens | phosphorylethanolamine + diacylglycerol | - |
? | |
3.1.4.3 | phosphatidylethanolamine + H2O | - |
Clostridium perfringens PB6K | phosphorylethanolamine + diacylglycerol | - |
? | |
3.1.4.3 | phosphatidylserine + H2O | - |
Clostridium perfringens | phosphorylserine + diacylglycerol | - |
? | |
3.1.4.3 | phosphatidylserine + H2O | - |
Clostridium perfringens PB6K | phosphorylserine + diacylglycerol | - |
? | |
3.1.4.3 | sphingomyelin + H2O | - |
Clostridium perfringens | ceramide + phosphorylcholine | - |
? | |
3.1.4.3 | sphingomyelin + H2O | - |
Clostridium perfringens PB6K | ceramide + phosphorylcholine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.3 | monomer | 31000-43000, gel filtration, SDS-PAGE | Clostridium perfringens |
3.1.4.3 | polymer | identical subunits of 31000-43000 Da, gel filtration, SDS-PAGE, multiple forms | Clostridium perfringens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.4.3 | 37 | - |
for 60 min, pH 5.0-10.0 | Clostridium perfringens |
3.1.4.3 | 60 | 70 | deactivation | Clostridium perfringens |
3.1.4.3 | 100 | - |
almost complete restoration of activity of enzyme deactivated at 60-70°C | Clostridium perfringens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.4.3 | 7 | 8 | substrate: phosphatidylcholine | Clostridium perfringens |