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Literature summary extracted from

  • Bahk, Y.Y.; Lee, Y.H.; Ryu, S.H.; Suh, P.G.
    Overexpression and biochemical characterization of phospholipase C-beta 1a and -beta 1b (1994), Mol. Cells, 4, 361-367.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.3 sodium deoxycholate 1mg/ml Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.3 transfected into CV-1 cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 Ca2+ slightly inhibitory at concentrations of 10 mM Rattus norvegicus
3.1.4.3 sodium deoxycholate at concentrations above 1mg/ml Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 0.087
-
 phosphatidylinositol PLC-beta1a, 2mM free Ca2+ Rattus norvegicus
3.1.4.3 0.137
-
 phosphatidylinositol 4,5-bisphosphate PLC-beta1a, 2mM free Ca2+ Rattus norvegicus
3.1.4.3 0.369
-
 phosphatidylinositol PLC-beta1b, 2mM free Ca2+ Rattus norvegicus
3.1.4.3 0.571
-
 phosphatidylinositol 4,5-bisphosphate PLC-beta1b, 2mM free Ca2+ Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Ca2+ PLC-beta 1a and -beta 1b: maximum activity with 3 mM with phosphatidylinositol for substrate, PLC translocation to membranes by free Ca2+ concentration of 100 nM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 140000
-
 recombinant PLC -beta 1b, SDS-PAGE Rattus norvegicus
3.1.4.3 150000
-
 recombinant PLC -beta 1a, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylinositol-bisphosphate + H2O Rattus norvegicus
-
 inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3 recombinant PLC-beta 1a 58fold, recombinant PLC-beta 1b 47fold, HPLC, affinity chromatography, reverse phase chromatography Rattus norvegicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 0.78
-
 PLC-beta 1b, substrate: phosphatidylinositol Rattus norvegicus
3.1.4.3 1.34
-
 PLC-beta 1a, substrate: phosphatidylinositol Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 phosphatidylinositol + H2O
-
 Rattus norvegicus inositol monophosphate + diacylglycerol
-
 ?
3.1.4.3 phosphatidylinositol-bisphosphate + H2O
-
 Rattus norvegicus inositol 1,4,5-trisphosphate + 1,2-diacylglycerol
-
 ?